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采用被动血凝试验检测感染猪疥螨变种的猪血清中抗猪疥螨抗原的抗体。

Detection of serum antibodies to sarcoptic mange mite antigens by the passive hemagglutination assay in pigs infested with Sarcoptes scabiei var. suis.

作者信息

Wooten E L, Gaafar S M

出版信息

Vet Parasitol. 1984 Sep;15(3-4):309-16. doi: 10.1016/0304-4017(84)90083-9.

Abstract

Development and remission of the clinical signs of sarcoptic mange in pigs experimentally infested once and twice with Sarcoptes scabiei var. suis were described. Encrusted lesions in the ears of pigs and a general dermatitis were observed 3 weeks after infestation, but subsided gradually. Specific antibody activity, in titers averaging 1:16 000 in sera from rabbits immunized with mite extracts and 1:512 in the serum from a pig artificially infested with sarcoptic manage and then twice immunized with mite extract, was detected with the passive hemagglutination assay with tanned bovine red blood cells. Titers ranging from 1:40 to 1:160 were detected in sera from experimentally-infested animals. Only 3 of 75 sera from chronically naturally-infested pigs exhibited antibody titers of 1:40 to the mite extract.

摘要

描述了猪实验性感染一次和两次猪疥螨后猪疥螨病临床症状的发展和缓解情况。感染后3周观察到猪耳部出现结痂病变和全身性皮炎,但逐渐消退。用鞣酸处理的牛红细胞进行被动血凝试验检测到特异性抗体活性,用螨提取物免疫的兔血清中抗体效价平均为1:16000,人工感染疥螨病后再用螨提取物免疫两次的猪血清中抗体效价为1:512。在实验感染动物的血清中检测到的效价范围为1:40至1:160。在长期自然感染猪的75份血清中,只有3份对螨提取物的抗体效价为1:40。

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