Morgan M S, Arlian L G
Department of Biological Sciences, Wright State University, Dayton, OH 45435.
Folia Parasitol (Praha). 1994;41(3):223-7.
The circulating antibody profiles of rabbits infected or immunized with Sarcoptes scabiei var. canis were compared. Crossed immuno-electrophoretic analysis showed that infested hosts produced serum antibody to 12 proteins (antigens) in an extract made from sarcoptic mite bodies. In contrast, rabbits immunized with an extract made from mite bodies produced antibody to 20 Sarcoptes proteins (antigens). SDS-PAGE/immunoblot analysis revealed that serum from immunized rabbits contained antibodies that bound strongly to proteins of 25 and 39-52 kD that were only barely visualized by antibodies in serum from infested rabbits.
对感染或免疫犬疥螨的家兔的循环抗体谱进行了比较。交叉免疫电泳分析表明，受感染的宿主针对从疥螨虫体提取物中的12种蛋白质（抗原）产生血清抗体。相比之下，用螨体提取物免疫的家兔针对20种疥螨蛋白质（抗原）产生抗体。SDS-PAGE/免疫印迹分析显示，免疫家兔的血清中含有与25 kD和39 - 52 kD蛋白质强烈结合的抗体，而受感染家兔血清中的抗体只能勉强识别这些蛋白质。
