流产布鲁氏菌凝集抗体和非凝集抗体的分析及体内检测
Analysis and in vivo assay of B. abortus agglutinating and non-agglutinating antibodies.
作者信息
Parma A E, Santisteban G, Margni R A
出版信息
Vet Microbiol. 1984 Aug;9(4):391-8. doi: 10.1016/0378-1135(84)90008-7.
Studies were made of physicochemical and immunochemical characteristics of Brucella abortus agglutinating and non-agglutinating antibodies in the sera of cattle repeatedly injected with living B. abortus (Strain 1119). Both agglutinating and non-agglutinating antibody were shown to be IgG1, and by immunodiffusion against rabbit anti-cattle gamma-globulin, agglutinating antibody gave a precipitation line of identity with that given by non-agglutinating antibody. Whilst agglutinating antibody increased clearance of antigen from the blood of passively protected mice, non-agglutinating antibody did not enhance clearance. Determination of the spleen infection index in mice pre-treated with agglutinating and non-agglutinating antibody showed that in animals passively immunized with non-agglutinating antibody the number of living (infecting) bacteria was approximately 4 times higher than in the case of agglutinating antibody. The possible potentiation of chronic B. abortus infection by non-agglutinating antibody is discussed.
对反复接种活布鲁氏菌流产亚种（菌株1119）的牛血清中布鲁氏菌流产亚种凝集性和非凝集性抗体的理化及免疫化学特性进行了研究。结果表明，凝集性抗体和非凝集性抗体均为IgG1，通过与兔抗牛γ球蛋白进行免疫扩散试验，凝集性抗体与非凝集性抗体产生了相同的沉淀线。虽然凝集性抗体可增加被动保护小鼠血液中抗原的清除率，但非凝集性抗体并未增强清除作用。对用凝集性和非凝集性抗体预处理的小鼠脾脏感染指数的测定表明，在用非凝集性抗体进行被动免疫的动物中，活菌（感染菌）数量比用凝集性抗体的情况高出约4倍。文中讨论了非凝集性抗体可能增强布鲁氏菌流产亚种慢性感染的问题。
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