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牛流产布鲁氏菌抗体放射免疫测定法：一种用于牛布鲁氏菌病的新型血清学检测方法。

Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis.

作者信息

Chappel R J, Williamson P, McNaught D J, Dalling M J, Allan G S

出版信息

J Hyg (Lond). 1976 Dec;77(3):369-76. doi: 10.1017/s0022172400055741.

DOI:10.1017/s0022172400055741
PMID:826587
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2129824/
Abstract

A radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to IgM, a characteristic which may make it more suitable than the complement fixation test (CFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

摘要

已开发出一种放射免疫测定法（RIA）来检测牛血清中抗流产布鲁氏菌的抗体，可用于牛布鲁氏菌病的诊断。该放射免疫测定法可测量IgG1和IgG2亚类的特异性抗体量，但对IgM不敏感，这一特性可能使其比补体结合试验（CFT）或血清凝集试验更适合区分感染动物与接种过流产布鲁氏菌19号菌株的动物。放射免疫测定法不会出现前带现象或模糊反应，而这两种情况都会干扰补体结合试验的结果判读。

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Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis.牛流产布鲁氏菌抗体放射免疫测定法：一种用于牛布鲁氏菌病的新型血清学检测方法。
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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Studies on a nonspecific Brucella-agglutinating substance in bovin serum. I. The differentiation of the specific and nonspecific agglutinins by heat treatment.牛血清中一种非特异性布鲁氏菌凝集物质的研究。I. 通过热处理区分特异性和非特异性凝集素。
Am J Vet Res. 1953 Apr;14(51):192-4.
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Immunity to brucella infection.布鲁氏菌感染免疫
Medicine (Baltimore). 1973 Jul;52(4):339-56. doi: 10.1097/00005792-197307000-00013.
4
An enzymic method for the trace iodination of immunoglobulins and other proteins.一种用于免疫球蛋白及其他蛋白质微量碘化的酶法。
Biochem J. 1969 Jun;113(2):299-305. doi: 10.1042/bj1130299.
5
The effect of natural infection with Salmonella urbana on the serological status of cattle in relation to tests for brucellosis.城市沙门氏菌自然感染对牛血清学状态及布鲁氏菌病检测的影响。
Br Vet J. 1975 May-Jun;131(3):324-34. doi: 10.1016/s0007-1935(17)35289-2.
6
A quantitative comparison of the sensitivity of serological test for bovine brucellosis to different antibody classes.牛布鲁氏菌病血清学检测对不同抗体类别的敏感性定量比较。
J Hyg (Lond). 1976 Apr;76(2):287-98. doi: 10.1017/s0022172400055182.
7
A mechanism for prozone formation in the complement fixation test for bovine brucellosis.牛布鲁氏菌病补体结合试验中前带形成的机制。
Aust Vet J. 1975 Aug;51(8):374-7. doi: 10.1111/j.1751-0813.1975.tb15598.x.
8
The serological diagnosis of bovine brucellosis: an evaluation of the complement fixation, serum agglutination and rose bengal tests.牛布鲁氏菌病的血清学诊断：补体结合试验、血清凝集试验及孟加拉玫瑰红试验的评估
Aust Vet J. 1975 Feb;51(2):57-63. doi: 10.1111/j.1751-0813.1975.tb09404.x.

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