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用于鉴定马尿的对流免疫电泳。

Counterimmunoelectrophoresis for identification of equine urine.

作者信息

Edwards K E, Stevens S, Woodward C B, Tweeten K A

出版信息

Am J Vet Res. 1984 Sep;45(9):1818-21.

PMID:6437304
Abstract

Counterimmunoelectrophoresis was evaluated as a method to distinguish urine of human origin from that of equine origin. The procedure used anti-equine serum and anti-human serum antibodies that had been solid-phase absorbed to eliminate species cross-reactivity. Counterimmunoelectrophoresis reliably detected contamination of equine urine by human urine to a level of 10% with a minimum sensitivity to about 2% contamination. Compared with double diffusion, counterimmunoelectrophoresis was approximately 10 to 15 times more sensitive in the detection of urine proteins.

摘要

对流免疫电泳被评估为一种区分人源尿液和马源尿液的方法。该程序使用已进行固相吸收以消除物种交叉反应性的抗马血清和抗人血清抗体。对流免疫电泳能够可靠地检测出人尿对马尿的污染,污染水平低至10%,最低检测灵敏度约为2%的污染。与双向扩散相比,对流免疫电泳在检测尿蛋白方面的灵敏度大约高10至15倍。

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