Ostman J, Arner P, Kimura H, Wahrenberg H, Engfeldt P
Eur J Clin Invest. 1984 Oct;14(5):383-91. doi: 10.1111/j.1365-2362.1984.tb01199.x.
The influence of 1 week's total fasting on the lipolytic effect of adrenergic agonists and on the binding of adrenergic antagonists was examined in isolated adipocytes of subcutaneous specimens removed from the hypogastric and the femoral sites in seventeen obese women. In the femoral adipocytes the lipolytic sensitivity to isopropyl noradrenaline decreased 30-fold (P less than 0.01) during fasting. The specific binding of the radioligands (-)-[3H]-dihydroalprenolol and (-)-[125I]-cyanopindolol decreased significantly during fasting, essentially owing to a reduction in the receptor density. In adipocytes from the hypogastric region no such changes were found. For both tissue regions fasting induced a right-ward shift in the dose-response curve for the inhibitory effect of the alpha 2 agonist, clonidine, on theophylline-induced lipolysis, corresponding to a 10-fold decrease in sensitivity. There was also a significant decrease of about 20% in the alpha 2-adrenoceptor density, as estimated with the radioligand [3H]-yohimbine. The results suggest that the regulation of the lipid mobilization in man by the sympathetic nervous system during fasting occurs not only through an increase in the level of circulating noradrenaline but also through changes in the adrenergic receptor density of the adipocytes.
在17名肥胖女性的下腹部和股部皮下分离的脂肪细胞中，研究了1周完全禁食对肾上腺素能激动剂的脂解作用及肾上腺素能拮抗剂结合的影响。在禁食期间，股部脂肪细胞对异丙基去甲肾上腺素的脂解敏感性降低了30倍（P<0.01）。放射性配体（-）-[3H]-二氢阿普洛尔和（-）-[125I]-氰胍心安的特异性结合在禁食期间显著降低，主要是由于受体密度降低。在下腹部区域的脂肪细胞中未发现此类变化。对于两个组织区域，禁食导致α2激动剂可乐定对茶碱诱导的脂解抑制作用的剂量反应曲线向右移动，敏感性降低了10倍。用放射性配体[3H]-育亨宾估计，α2-肾上腺素能受体密度也显著降低约20%。结果表明，禁食期间人体交感神经系统对脂质动员的调节不仅通过循环去甲肾上腺素水平的升高，还通过脂肪细胞肾上腺素能受体密度的变化来实现。
