Caroff M, Bundle D R, Perry M B, Cherwonogrodzky J W, Duncan J R
Infect Immun. 1984 Nov;46(2):384-8. doi: 10.1128/iai.46.2.384-388.1984.
Antigenic phenol-phase soluble lipopolysaccharide isolated from Brucella abortus 1119-3 by hot phenol-water extraction was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, controlled hydrolysis, periodate oxidation, methylation, and 1H and 13C nuclear magnetic resonance studies to be an S-type lipopolysaccharide which could be cleaved to yield a lipid A and an O-chain polysaccharide identified as an unbranched linear homopolymer of 1,2-linked 4,6-dideoxy-4-formamido-alpha-D-mannopyranosyl residues. The serological reactivity of bovine antiserum to B. abortus 1119-3 with the lipopolysaccharides of Yersinia enterocolitica serotype O:9 and Vibrio cholerae species has now been related to the occurrence of 1,2-linked N-acylated 4-amino-4,6-dideoxy-alpha-D-mannopyranosyl units in the O-chain polysaccharides of their lipopolysaccharides.
通过热酚水萃取从流产布鲁氏菌1119 - 3中分离出的抗原性酚相可溶性脂多糖，经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、控制水解、高碘酸盐氧化、甲基化以及1H和13C核磁共振研究表明，它是一种S型脂多糖，可裂解产生脂质A和O链多糖，该O链多糖被鉴定为1,2 - 连接的4,6 - 二脱氧 - 4 - 甲酰胺基 - α - D - 甘露吡喃糖残基的无分支线性均聚物。牛抗流产布鲁氏菌1119 - 3血清与小肠结肠炎耶尔森菌O:9血清型和霍乱弧菌脂多糖的血清学反应性，现已与它们脂多糖O链多糖中1,2 - 连接的N - 酰化4 - 氨基 - 4,6 - 二脱氧 - α - D - 甘露吡喃糖单元的存在相关。
