Kane M A, Bradley D W, Shrestha S M, Maynard J E, Cook E H, Mishra R P, Joshi D D
JAMA. 1984 Dec 14;252(22):3140-5. doi: 10.1001/jama.252.22.3140.
An epidemic of non-A, non-B hepatitis occurred in Kathmandu Valley, Nepal, during 1981-1982, with approximately 7.6% of households and 1.4% of individuals affected. Cases occurred preponderantly in the 15- to 34-year-old age group (70%), with most cases (75%) occurring in males. A high mortality rate (21%) occurred in pregnant women admitted to the hospital. No single water source was implicated, but epidemic peaks occurred during monsoon rains, and multiple opportunities for enteric transmission existed. One of eight patient stools examined by immune electron microscopy revealed aggregated, antibody-coated, 27-nm viruslike particles when convalescent serum samples were used as sources of antibody. Inoculation of two chimpanzees and four marmosets with a suspension of this stool resulted in elevated liver enzyme activity in three marmosets. Fecal excretion of 27-nm particles during the acute phase of disease (with temporally coincident antigen activity by radioimmunoassay) was observed in one marmoset, which also developed convalescent antibody against the particles in the original inoculum.
1981年至1982年期间，尼泊尔加德满都谷地爆发了非甲非乙型肝炎疫情，约7.6%的家庭和1.4%的个人受到影响。病例主要发生在15至34岁年龄组（70%），大多数病例（75%）为男性。入院的孕妇死亡率很高（21%）。没有单一水源被认为与此有关，但疫情高峰出现在季风降雨期间，存在多种肠道传播机会。当使用恢复期血清样本作为抗体来源时，通过免疫电子显微镜检查的8份患者粪便中有1份显示出聚集的、被抗体包被的27纳米病毒样颗粒。用这种粪便悬液接种两只黑猩猩和四只狨猴，三只狨猴的肝酶活性升高。在一只狨猴中观察到在疾病急性期粪便中排出27纳米颗粒（同时通过放射免疫测定有抗原活性），该狨猴还产生了针对原始接种物中颗粒的恢复期抗体。
