嗜酸乳杆菌细菌素lactacin B的纯化与特性分析
Purification and characterization of the Lactobacillus acidophilus bacteriocin lactacin B.
作者信息
Barefoot S F, Klaenhammer T R
出版信息
Antimicrob Agents Chemother. 1984 Sep;26(3):328-34. doi: 10.1128/AAC.26.3.328.
Abstract
Parameters for production and purification of a bacteriocin produced by Lactobacillus acidophilus N2 are described. Production of lactacin B was pH dependent, with maximum activity detected in broth cultures maintained at pH 6. Lactacin B was purified by ion-exchange chromatography, ultrafiltration, and successive gel filtrations in the presence of 8 M urea and then 0.1% sodium dodecyl sulfate. The molecular weight of lactacin B was ca. 6,000 to 6,500, and the purified compound showed maximum absorbance at 211 nm. The activity of purified lactacin B was bactericidal to sensitive cells and restricted to members of the family Lactobacilliaceae, L. leichmannii, L. bulgaricus, L. helveticus, and L. lactis. Characteristics identified for lactacin B indicated that it was a peptide and confirmed its identity as a bacteriocin.
摘要
描述了嗜酸乳杆菌N2产生的一种细菌素的生产和纯化参数。乳酸乳球菌素B的产生依赖于pH值，在pH 6的肉汤培养物中检测到最大活性。通过离子交换色谱、超滤以及在8 M尿素存在下随后在0.1%十二烷基硫酸钠存在下的连续凝胶过滤来纯化乳酸乳球菌素B。乳酸乳球菌素B的分子量约为6000至6500，纯化后的化合物在211 nm处显示最大吸光度。纯化的乳酸乳球菌素B对敏感细胞具有杀菌活性，且仅限于乳杆菌科的成员，如leichmannii乳杆菌、保加利亚乳杆菌、瑞士乳杆菌和乳酸乳球菌。鉴定出的乳酸乳球菌素B的特性表明它是一种肽，并证实了其作为细菌素的身份。
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