Otto-Verberne C J, Ten Have-Opbroek A A, Balkema J J, Franken C
Department of Anatomy and Embryology, University of Leiden, The Netherlands.
Anat Embryol (Berl). 1988;178(1):29-39. doi: 10.1007/BF00305011.
The present study was performed to find out whether the type II alveolar epithelial cell or its precursor (an approximately cuboidal cell lacking multilamellar bodies) is present before the twentieth week of human gestation. For this purpose we used an antibody, SALS-Hu(E), which recognizes the human type II cell on the basis of surfactant-associated proteins. Application of SALS-HuE (by indirect immunofluorescence) to acetone-fixed frozen sections of fetal lung tissue gave a distinct staining of the cuboidal or low columnar epithelial cells lining the end-pieces of the tubular system of fetal lung (initially only a few): this staining started around weeks 10 to 12 after conception. Around week 16 some of the labeled epithelial cells appeared to be rather flat and by week 19 a combined cellular and linear fluorescence pattern was seen. Columnar epithelial cells of the prospective bronchial portion did not show this specific staining. Our results indicate that the type II cell or its precursor cell is indeed present in the pseudoglandular period of human lung development, i.e., starting around the tenth to twelfth week. This cell type lines the acinar tubule, the basic structure of the pulmonary acinus. Transformation of this cell type into the type I alveolar epithelial cell seems to start in week 16.
本研究旨在确定在人类妊娠20周之前是否存在Ⅱ型肺泡上皮细胞或其前体细胞（一种缺乏板层小体的近似立方形细胞）。为此，我们使用了一种抗体SALS-Hu(E)，它基于表面活性物质相关蛋白识别人类Ⅱ型细胞。将SALS-HuE（通过间接免疫荧光法）应用于胎儿肺组织的丙酮固定冰冻切片，可使胎儿肺管状系统终末部分内衬的立方形或低柱状上皮细胞呈现明显的染色（最初只有少数细胞）：这种染色在受孕后10至12周左右开始。在16周左右，一些标记的上皮细胞似乎变得相当扁平，到19周时，可见细胞和线性荧光的组合模式。预期支气管部分的柱状上皮细胞未显示这种特异性染色。我们的结果表明，Ⅱ型细胞或其前体细胞确实存在于人类肺发育的假腺期，即大约从第10至12周开始。这种细胞类型排列在腺泡小管内，而腺泡小管是肺腺泡的基本结构。这种细胞类型向Ⅰ型肺泡上皮细胞的转变似乎在16周开始。
