Cottle M K, Cottle W H, Pérusse F, Bukowiecki L J
Histochem J. 1985 Dec;17(12):1279-88. doi: 10.1007/BF01002525.
A glyoxylic acid method using cryostat sections to demonstrate catecholaminergic fibres of the central nervous system was modified to show the extent of the adrenergic innervation in rat brown adipose tissue. It revealed prominent interlacing fluorescent parenchymal fibres surrounding individual adipocytes. The density of this network of fine fibres was not evident using earlier techniques. The new method also confirmed the dense networks of adrenergic fibres associated with arterial vessels. Its specificity was verified by simultaneously performing radioenzymatic determinations of tissue catecholamine levels and histochemical studies of brown adipose tissue from normal and sympathectomized rats. Chemical sympathectomy with 6-hydroxydopamine resulted in a pronounced decrease in brown adipose tissue and heart catecholamine (noradrenalin and dopamine) levels. Significantly, in brown adipose tissue of sympathectomized animals no fluorescence could be detected in terminal nerves of either the parenchyma or those of vascular smooth muscles. Nevertheless, some intense fluorescence was seen in axon bundles. The findings suggest that catecholamines of the parenchymal innervation form a larger proportion of the total catecholamine content of brown adipose tissue than was previously believed, provide stronger support for direct control of the function of multilocular adipocytes, and also confirm unpublished data reporting considerable dopamine content in brown adipose tissue.
一种利用低温恒温器切片来显示中枢神经系统儿茶酚胺能纤维的乙醛酸法，经改进后用于显示大鼠棕色脂肪组织中肾上腺素能神经支配的范围。结果显示，单个脂肪细胞周围有明显的交织荧光实质纤维。使用早期技术时，这种细纤维网络的密度并不明显。新方法还证实了与动脉血管相关的肾上腺素能纤维的密集网络。通过同时对正常大鼠和交感神经切除大鼠的棕色脂肪组织进行组织儿茶酚胺水平的放射酶法测定和组织化学研究，验证了其特异性。用6-羟基多巴胺进行化学交感神经切除导致棕色脂肪组织和心脏儿茶酚胺（去甲肾上腺素和多巴胺）水平显著降低。值得注意的是，在交感神经切除动物的棕色脂肪组织中，实质或血管平滑肌的终末神经中均未检测到荧光。然而，在轴突束中可见一些强烈荧光。这些发现表明，实质神经支配中的儿茶酚胺在棕色脂肪组织总儿茶酚胺含量中所占比例比以前认为的要大，为多泡脂肪细胞功能的直接控制提供了更强有力的支持，也证实了未发表的数据，即棕色脂肪组织中含有相当数量的多巴胺。
