对由T细胞衍生的淋巴因子诱导分泌IgG的B细胞进行克隆分析。
Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s).
作者信息
Layton J E, Vitetta E S, Uhr J W, Krammer P H
出版信息
J Exp Med. 1984 Dec 1;160(6):1850-63. doi: 10.1084/jem.160.6.1850.
Abstract
To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1-secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion.
摘要
为深入了解T细胞衍生的淋巴因子如何诱导活化B细胞分泌IgG，我们进行了有限稀释分析，使用与脂多糖（LPS）和含有T细胞衍生的IgG B细胞分化因子（BCDFγ）的上清液（SN）一起孵育的小鼠脾脏B细胞。该分析结果表明，这样的SN可诱导分泌IgG1的细胞前体频率显著增加，克隆大小适度增加。这些前体缺乏表面IgG，在LPS激活后但在添加含BCDFγ的SN之前就已确定进入IgG1分泌的分化途径。大多数分泌IgG1的克隆独立来源于分泌IgG3的细胞前体。综上所述，这些结果表明BCDFγ指导活化B细胞分化为分泌IgG1。
相似文献
1
Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s).对由T细胞衍生的淋巴因子诱导分泌IgG的B细胞进行克隆分析。
J Exp Med. 1984 Dec 1;160(6):1850-63. doi: 10.1084/jem.160.6.1850.
2
3
Isotype switching in anti-immunoglobulin-activated B lymphoblasts: differential requirements for interleukin 4 and other lymphokines to elicit membrane vs. secreted IgG1.抗免疫球蛋白激活的B淋巴母细胞中的同种型转换：诱导膜结合型与分泌型IgG1产生对白介素4和其他淋巴因子的不同需求。
Eur J Immunol. 1991 Mar;21(3):707-14. doi: 10.1002/eji.1830210325.
4
Lymphokine-like activity of 8-mercaptoguanosine: induction of T and B cell differentiation.8-巯基鸟苷的淋巴细胞因子样活性：诱导T细胞和B细胞分化。
J Immunol. 1985 May;134(5):3204-11.
5
6
7
8
Immunoglobulin C-gene expression. III. Possible induction of specific genetic events in activated B lymphocytes by the polyclonal stimuli driving clonal expansion.免疫球蛋白C基因表达。III. 驱动克隆扩增的多克隆刺激可能在活化B淋巴细胞中诱导特定的基因事件。
Eur J Immunol. 1982 Jun;12(6):502-6. doi: 10.1002/eji.1830120610.
9
Identification of a B cell differentiation factor(s) spontaneously produced by proliferating T cells in murine lupus strains of the lpr/lpr genotype.在lpr/lpr基因型的小鼠狼疮品系中，鉴定增殖T细胞自发产生的B细胞分化因子。
J Exp Med. 1983 Feb 1;157(2):730-42. doi: 10.1084/jem.157.2.730.
10
Induction of IgG2a secretion from mIgG2a+ B-lymphoid tumor cells by BCDF present in several antigen-specific T-helper clones.几种抗原特异性辅助性T细胞克隆中存在的BCDF可诱导mIgG2a+B淋巴细胞瘤细胞分泌IgG2a。
Cell Immunol. 1987 Nov;110(1):149-62. doi: 10.1016/0008-8749(87)90109-2.
引用本文的文献
1
The role of germline promoters and I exons in cytokine-induced gene-specific class switch recombination.胚系启动子和 I 外显子在细胞因子诱导的基因特异性类别转换重组中的作用。
J Immunol. 2011 Jan 1;186(1):350-8. doi: 10.4049/jimmunol.1003108. Epub 2010 Dec 3.
2
H3 trimethyl K9 and H3 acetyl K9 chromatin modifications are associated with class switch recombination.H3 赖氨酸 9 三甲基化和 H3 赖氨酸 9 乙酰化染色质修饰与类别转换重组相关。
Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5288-93. doi: 10.1073/pnas.0901368106. Epub 2009 Mar 10.
3
An anti-CD45RO immunotoxin kills HIV-latently infected cells from individuals on HAART with little effect on CD8 memory.一种抗CD45RO免疫毒素可杀死接受高效抗逆转录病毒治疗（HAART）的个体中潜伏感染HIV的细胞，而对CD8记忆细胞影响很小。
Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2494-9. doi: 10.1073/pnas.0308381100.
4
Class switching in B cells lacking 3' immunoglobulin heavy chain enhancers.缺乏3'免疫球蛋白重链增强子的B细胞中的类别转换。
J Exp Med. 1998 Oct 19;188(8):1421-31. doi: 10.1084/jem.188.8.1421.
5
Interleukin-9 potentiates the interleukin-4-induced IgE and IgG1 release from murine B lymphocytes.白细胞介素-9增强白细胞介素-4诱导的小鼠B淋巴细胞释放IgE和IgG1。
Immunology. 1993 May;79(1):146-51.
6
A selective defect in IgG2b switching as a result of targeted mutation of the I gamma 2b promoter and exon.由于Igamma 2b启动子和外显子的靶向突变导致IgG2b转换的选择性缺陷。
EMBO J. 1993 Sep;12(9):3529-37. doi: 10.1002/j.1460-2075.1993.tb06027.x.
7
Serological, biochemical, and functional identity of B cell-stimulatory factor 1 and B cell differentiation factor for IgG1.B细胞刺激因子1与IgG1的B细胞分化因子的血清学、生化及功能特性
J Exp Med. 1985 Nov 1;162(5):1726-31. doi: 10.1084/jem.162.5.1726.
8
B cell stimulatory factor 1 (BSF-1) prepares resting B cells to enter S phase in response to anti-IgM and lipopolysaccharide.B细胞刺激因子1（BSF-1）使静止B细胞做好准备，以响应抗IgM和脂多糖进入S期。
J Exp Med. 1986 Aug 1;164(2):517-31. doi: 10.1084/jem.164.2.517.
9
Class switch recombination is IgG1 specific on active and inactive IgH loci of IgG1-secreting B-cell blasts.类别转换重组在分泌IgG1的B细胞母细胞的活性和非活性IgH基因座上具有IgG1特异性。
Proc Natl Acad Sci U S A. 1986 Jun;83(11):3954-7. doi: 10.1073/pnas.83.11.3954.
10
Interleukin 4 causes isotype switching to IgE in T cell-stimulated clonal B cell cultures.白细胞介素4在T细胞刺激的克隆B细胞培养物中导致同型转换为IgE。
J Exp Med. 1988 Sep 1;168(3):853-62. doi: 10.1084/jem.168.3.853.
本文引用的文献
1
T cell-derived B cell differentiation factor(s). Effect on the isotype switch of murine B cells.T细胞衍生的B细胞分化因子。对小鼠B细胞同种型转换的影响。
J Exp Med. 1982 Mar 1;155(3):734-48. doi: 10.1084/jem.155.3.734.
2
Human T cell hybridomas secreting factors for IgA-specific help, polyclonal B cell activation, and B cell proliferation.分泌针对IgA特异性辅助、多克隆B细胞活化和B细胞增殖因子的人T细胞杂交瘤。
J Exp Med. 1982 Dec 1;156(6):1860-5. doi: 10.1084/jem.156.6.1860.
3
T cell regulation of IgG subclass antibody production in response to T-independent antigens.T细胞对非T细胞依赖性抗原产生的IgG亚类抗体产生的调节作用。
J Exp Med. 1981 Jan 1;153(1):1-12. doi: 10.1084/jem.153.1.1.
4
Subclass restriction of murine antibodies. II. The IgG plaque-forming cell response to thymus-independent type 1 and type 2 antigens in normal mice and mice expressing an X-linked immunodeficiency.小鼠抗体的亚类限制。II. 正常小鼠和表达X连锁免疫缺陷的小鼠对1型和2型非胸腺依赖性抗原的IgG斑块形成细胞反应。
J Exp Med. 1980 Apr 1;151(4):853-62. doi: 10.1084/jem.151.4.853.
5
Rearrangements between the immunoglobulin heavy chain gene JH and C mu regions accompany normal B lymphocyte differentiation in vitro.免疫球蛋白重链基因JH和Cμ区域之间的重排伴随着体外正常B淋巴细胞的分化。
Nature. 1982 Oct 21;299(5885):742-4. doi: 10.1038/299742a0.
6
Expression of surface and secreted IgG2a by a murine B-lymphoma before and after hybridization to myeloma cells.小鼠B淋巴瘤细胞与骨髓瘤细胞杂交前后表面及分泌型IgG2a的表达
Mol Immunol. 1981 Apr;18(4):311-22. doi: 10.1016/0161-5890(81)90055-9.
7
Deletion of immunoglobulin heavy chain genes from expressed allelic chromosome.从表达的等位染色体上删除免疫球蛋白重链基因。
Nature. 1980 Aug 28;286(5776):850-3. doi: 10.1038/286850a0.
8
9
The role of gene deletion in the immunoglobulin heavy chain switch.基因缺失在免疫球蛋白重链转换中的作用。
Nature. 1980 Jan 24;283(5745):351-6. doi: 10.1038/283351a0.
10
A simple semi-automated plaque method for the detection of antibody-forming cell clones in microcultures.一种用于检测微量培养物中抗体形成细胞克隆的简单半自动蚀斑法。
J Immunol Methods. 1982;52(1):25-37. doi: 10.1016/0022-1759(82)90346-5.
Zotero 插件