Rabin E M, Mond J J, Ohara J, Paul W E
J Exp Med. 1986 Aug 1;164(2):517-31. doi: 10.1084/jem.164.2.517.
BSF-1 prepares resting BALB/c, DBA/2, and BDF1 B cells to enter S phase more promptly in response to subsequent culture with anti-IgM-based stimulants. It prepares DBA/2 and BDF1 B cells to respond to LPS, but its preparative effect for LPS responses of BALB/c B cells is both inconstant and meager. Preparation mediated by BSF-1 requires extended contact of B cells with the stimulant for full effect. Half-maximal preparation requires approximately 12 h of contact, as judged by delayed addition of BSF-1 or by inhibition of BSF-1 action with anti-BSF-1 mAbs. BSF-1 preparative action on resting DBA/2 B cells is mimicked by anti-Lyb-2.1 antibody. B cell blasts prepared by culture with BSF-1 and anti-IgM show modest responses to high concentrations of BSF-1; large B cells directly isolated from the spleen are not stimulated to enter S phase by BSF-1. These results lead us to conclude that BSF-1 functions principally as an activation factor for resting B cells.
BSF-1使静止的BALB/c、DBA/2和BDF1 B细胞做好准备,以便在随后用基于抗IgM的刺激物培养时更迅速地进入S期。它使DBA/2和BDF1 B细胞对LPS产生反应,但其对BALB/c B细胞LPS反应的预处理作用既不稳定也很微弱。由BSF-1介导的预处理需要B细胞与刺激物长时间接触才能产生充分效果。通过延迟添加BSF-1或用抗BSF-1单克隆抗体抑制BSF-1的作用来判断,半最大预处理需要大约12小时的接触。抗Lyb-2.1抗体可模拟BSF-1对静止DBA/2 B细胞的预处理作用。用BSF-1和抗IgM培养制备的B细胞母细胞对高浓度的BSF-1有适度反应;直接从脾脏分离的大B细胞不会被BSF-1刺激进入S期。这些结果使我们得出结论,BSF-1主要作为静止B细胞的激活因子发挥作用。