Rowland E C, Ritter D M
J Parasitol. 1984 Oct;70(5):760-6.
Treatment of susceptible C3H(He) mice with 10(7) live Corpus Christi strain culture-derived Trypanosoma cruzi provided protection against a subsequent Brazil strain challenge. This protection was indicated by a greater than 10-fold decrease in parasitemia and an increase in longevity (including survival) in many groups. The Corpus Christi organisms were unable to establish an apparent infection, but viability is an important element in the treatment in that freeze-thawed, non-viable preparations of the Corpus Christi strain were unable to provide protection. Adoptive transfer of resistance was achieved with spleen cells from Corpus Christi-treated, Brazil-infected mice which had recovered from the acute phase of infection. The T cell-depleted population of these spleen cells was able to transfer resistance whereas the T cell-enriched population was not protective. Passive transfer of serum from Corpus Christi-treated and Brazil-infected mice provided a temporary decrease in parasitemia in infected mice. The results presented herein suggest that Corpus Christi-induced protection to virulent T. cruzi challenge is mediated by antibody mechanisms.
用10⁷个源自科珀斯克里斯蒂菌株培养物的活克氏锥虫对易感的C3H(He)小鼠进行治疗，可使其免受随后巴西菌株攻击的影响。许多组中寄生虫血症下降超过10倍以及寿命（包括存活率）增加表明了这种保护作用。科珀斯克里斯蒂菌株的生物体无法建立明显的感染，但活力是治疗中的一个重要因素，因为科珀斯克里斯蒂菌株的冻融后无活力制剂无法提供保护。用来自已从感染急性期恢复的经科珀斯克里斯蒂治疗且感染巴西菌株的小鼠的脾细胞实现了抗性的过继转移。这些脾细胞中耗尽T细胞的群体能够转移抗性，而富含T细胞的群体则没有保护作用。经科珀斯克里斯蒂治疗且感染巴西菌株的小鼠的血清被动转移使感染小鼠的寄生虫血症暂时降低。本文给出的结果表明，科珀斯克里斯蒂诱导的对强毒克氏锥虫攻击的保护作用是由抗体机制介导的。
