恒河猴促性腺激素的放射免疫测定法。

Radioimmunoassay for rhesus monkey gonadotropins.

作者信息

Faiman C, Stearns E L, Winter J S, Reyes F I, Hobson W C

出版信息

Proc Soc Exp Biol Med. 1975 Jul;149(3):670-6. doi: 10.3181/00379727-149-38876.

Abstract

Heterologous double-antibody radioimmunoassay methods are described for the measurement of circulating levels of rhesus monkey (Macaca mulatta) FSH and LH; the latter assay is also applicable to rhesus chorionic gonadotropin (CG) estimations. The FSH assay utilizes purified rat FSH for trace, either of two anti-human FSH antisera and a semipurified rhesus pituitary standard. The LH assay utilizes purified ovine LH for trace, an anti-human CG antiserum and the same rhesus pituitary standard. The use of these systems obviates the necessity of purifying rhesus gonadotropins which are required for the development of homologous radioimmunoassay systems.

摘要

描述了用于测量恒河猴（猕猴）促卵泡激素（FSH）和促黄体生成素（LH）循环水平的异源双抗体放射免疫测定方法；后一种测定方法也适用于恒河猴绒毛膜促性腺激素（CG）的测定。FSH测定使用纯化的大鼠FSH作为示踪剂、两种抗人FSH抗血清中的一种以及半纯化的恒河猴垂体标准品。LH测定使用纯化的绵羊LH作为示踪剂、一种抗人CG抗血清和相同的恒河猴垂体标准品。使用这些系统避免了纯化同源放射免疫测定系统所需的恒河猴促性腺激素的必要性。