Hertz M M, Barry D I, Hemmingsen R, Bolwig T G
Acta Physiol Scand. 1984 Nov;122(3):397-400. doi: 10.1111/j.1748-1716.1984.tb07524.x.
It is assumed that the cerebral microcirculation is not perturbed by the intraarterial injection used in determination of cerebral blood flow (CBF) with the intraarterial 133Xenon technique (and in various assessments of blood brain barrier (BBB) permeability). The application of these techniques to the rat, where the injectate is large compared to normal blood flow, places this problem is focus and it has been claimed that since large intracarotid injections increased cerebral venous outflow, the CBF must also increase. We investigated this problem in the rat by means of the intraarterial 133Xenon injection technique, using a saline bolus injected in less than 1 sec and found that CBF was unaltered at bolus volumes between 10 and 100 microliters. Furthermore, injection of 100-200 microliters saline during washout detection did not change the slope of the semilogarithmic wash-put curves. It is concluded that in spite of large intracarotid injections the CBF remained constant and that the hemodilution produced by the saline bolus is not sufficient to influence CBF. Consequently, estimations of CBF yield valid results in the present rat preparation.
假定在使用动脉内注入133氙技术测定脑血流量（CBF）（以及在对血脑屏障（BBB）通透性的各种评估中）时，动脉内注射不会干扰脑微循环。将这些技术应用于大鼠时，与正常血流量相比，注入物量很大，这使得这个问题备受关注。有人声称，由于颈内动脉大量注射会增加脑静脉流出量，所以CBF也必然会增加。我们通过动脉内注入133氙技术对大鼠的这个问题进行了研究，使用在不到1秒内注入的生理盐水团注，发现当团注体积在10至100微升之间时，CBF没有改变。此外，在洗脱检测期间注入100 - 200微升生理盐水并没有改变半对数洗脱曲线的斜率。得出的结论是，尽管进行了颈内动脉大量注射，但CBF保持恒定，并且生理盐水团注产生的血液稀释不足以影响CBF。因此，在当前大鼠制备中对CBF的估计产生了有效的结果。
