Hiramine C, Hojo K
Am J Reprod Immunol (1980). 1984 Oct-Nov;6(3):85-91. doi: 10.1111/j.1600-0897.1984.tb00120.x.
The necessity of T lymphocytes in local adoptive transfer of experimental allergic orchitis (EAO) is an established fact. The present study was undertaken to elucidate the participation of B lymphocytes in the local transfer system of EAO. The capacity of purified lymph node T and B cells to transfer EAO was compared. Donor strain 13 guinea pigs were sensitized with testicular antigen and complete Freund's adjuvant. We then injected 1 X 10(8) cells just under the tunica albuginea of the testis of a syngeneic recipient. The recipients were killed 5 days after cell transfer. Nylon-wool nonadherent T cells alone produced only minimal lesions, but could induce more severe mononuclear lesions with the addition of syngeneic, normal macrophages. In contrast, B-cell enriched populations either alone or combined with a small number of macrophages, were able to transfer extensive lesions. In the combined transfer of immune T and immune B cells the severity of transferred lesions was dependent upon the number of B cells. These results suggest that cooperative interactions among T cells, B cells, and macrophages are involved in the generation of transferred testicular lesions, with emphasis on the participation of B cells.
T淋巴细胞在实验性变应性睾丸炎（EAO）局部过继转移中的必要性已是既定事实。本研究旨在阐明B淋巴细胞在EAO局部转移系统中的参与情况。比较了纯化的淋巴结T细胞和B细胞转移EAO的能力。用睾丸抗原和完全弗氏佐剂使供体品系13豚鼠致敏。然后将1×10⁸个细胞注射到同基因受体睾丸白膜下。细胞转移5天后处死受体。单独的尼龙毛非黏附T细胞仅产生轻微病变，但加入同基因正常巨噬细胞后可诱导更严重的单核细胞病变。相反，富含B细胞的群体单独或与少量巨噬细胞联合，都能够转移广泛的病变。在免疫T细胞和免疫B细胞的联合转移中，转移病变的严重程度取决于B细胞的数量。这些结果表明，T细胞、B细胞和巨噬细胞之间的协同相互作用参与了转移睾丸病变的产生，其中B细胞的参与尤为重要。
