Hojo K, Hiramine C, Ishitaki M
J Reprod Fertil. 1980 May;59(1):113-23. doi: 10.1530/jrf.0.0590113.
The development of cellular hypersensitivity was measured by the proliferative response of lymph node cells in vitro and a skin test to partly purified testicular antigen. There was a slight stimulation of lymph node cells and of the delayed cutaneous response at 1-2 weeks after sensitization with testicular antigen in Freund's complete adjuvant and then, after a decline in response, there was a pronounced peak at 6 weeks after sensitizaton. The early response coincided with the onset and active inflammatory stage of the effect on the testis, while the major peak was consistent with a state of aspermatogenesis. The proliferative response of unseparated lymph node cells could not be attributed to the function of T or B cells acting alone, although at 6 weeks after sensitization B-cell populations did become slightly responsive to antigen. The response of T lymphocytes was enhanced by the presence of B lymphocytes or peritoneal macrophages at 2 and 6 weeks after sensitization, but B cells did not appear to interact with macrophages. We conclude that T cells are essential to the proliferative response of lymph node cells during the induction of experimental allergic orchitis in guinea-pigs.
通过体外淋巴结细胞的增殖反应以及对部分纯化的睾丸抗原进行皮肤试验来测定细胞超敏反应的发展情况。在用弗氏完全佐剂中的睾丸抗原致敏后1 - 2周，淋巴结细胞和迟发性皮肤反应有轻微刺激，然后在反应下降后，致敏后6周出现明显峰值。早期反应与对睾丸影响的起始和活跃炎症阶段一致，而主要峰值与无精子发生状态相符。未分离的淋巴结细胞的增殖反应不能归因于单独作用的T细胞或B细胞的功能，尽管在致敏后6周B细胞群体确实对抗原稍有反应。在致敏后2周和6周，B淋巴细胞或腹腔巨噬细胞的存在增强了T淋巴细胞的反应，但B细胞似乎不与巨噬细胞相互作用。我们得出结论，在豚鼠实验性变应性睾丸炎诱导过程中，T细胞对于淋巴结细胞的增殖反应至关重要。
