Miyakawa M, Arai Y, Kikuyama S
Anat Embryol (Berl). 1984;170(2):113-5. doi: 10.1007/BF00318994.
In Bufo bufo japonicus catecholamine neurons in the preoptic recess organ (PRO) became detectable at the metamorphic climax by formaldehyde-induced fluorescence (Falck-Hillarp technique). In hypophysectomized tadpoles metamorphosis was inhibited and no fluorescent neurons appeared in the PRO. Implantation of a pituitary graft to the hypophysectomized tadpoles induced metamorphosis and development of PRO catecholamine neurons. Administration of corticosterone to hypophysectomized tadpoles resulted in the development of PRO catecholamine neurons in spite of the unmetamorphosed state. On the other hand, prolactin administration had no effect on the PRO neurons of hypophysectomized tadpoles. From these results, in conjunction with our previous results indicating that thyroxine treatment induces development of the PRO catecholamine neurons in thyroidectomized animals but not in hypophysectomized animals, it is concluded that corticosterone is a primary hormone for the development of PRO catecholamine neurons in toad tadpoles.
在日本蟾蜍中，视前隐窝器官（PRO）中的儿茶酚胺能神经元在变态高峰期通过甲醛诱导荧光法（Falck-Hillarp技术）可被检测到。在垂体切除的蝌蚪中，变态受到抑制，PRO中未出现荧光神经元。将垂体移植到垂体切除的蝌蚪中可诱导变态以及PRO儿茶酚胺能神经元的发育。给垂体切除的蝌蚪注射皮质酮，尽管处于未变态状态，但仍会导致PRO儿茶酚胺能神经元的发育。另一方面，注射催乳素对垂体切除的蝌蚪的PRO神经元没有影响。结合我们之前的结果，即甲状腺素处理可诱导甲状腺切除动物而非垂体切除动物的PRO儿茶酚胺能神经元发育，从这些结果可以得出结论，皮质酮是蟾蜍蝌蚪PRO儿茶酚胺能神经元发育的主要激素。
