Böhlen P, Wehrenberg W B, Esch F, Ling N, Brazeau P, Guillemin R
Biochem Biophys Res Commun. 1984 Dec 28;125(3):1005-12. doi: 10.1016/0006-291x(84)91383-4.
Growth hormone-releasing factor (GRF) was isolated from acid extracts of approximately 35,000 rat hypothalami by means of immunoaffinity chromatography, gel filtration and two steps of reverse-phase HPLC. Amino acid analysis, gas-liquid phases sequencing and peptide mapping established that rat GRF is a 43 amino acid peptide with the amino acid sequence His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly- Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-Gln-Gln-Gly- Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-OH, confirming the primary structure reported earlier (Spiess et al Nature 303, 532 (1983).
生长激素释放因子（GRF）是通过免疫亲和色谱、凝胶过滤和两步反相高效液相色谱法从约35,000个大鼠下丘脑的酸提取物中分离出来的。氨基酸分析、气液相测序和肽图谱分析确定大鼠GRF是一种由43个氨基酸组成的肽，其氨基酸序列为His-Ala-Asp-Ala-Ile-Phe-Thr-Ser-Ser-Tyr-Arg-Arg-Ile-Leu-Gly-Gln-Leu-Tyr-Ala-Arg-Lys-Leu-Leu-His-Glu-Ile-Met-Asn-Arg-Gln-Gln-Gly-Glu-Arg-Asn-Gln-Glu-Gln-Arg-Ser-Arg-Phe-Asn-OH，证实了先前报道的一级结构（斯皮斯等人，《自然》303, 532 (1983)）。
