Chekulaev V A, Lobanenkov V V, Karamysheva A F, Shelepov V P, Mironov N M
Biokhimiia. 1984 Sep;49(9):1515-22.
It was demonstrated that the DNA closely associated with the nuclear matrix preferentially binds 7,12-dimethylbenz(a)anthracene (DMBA) metabolites. In experiments with perfused rat liver, it was found that the kinetic curves for adduct formation in all DNA fractions of DNP have maxima, the largest differences in the rate of metabolite binding in the DNA fractions being observed at the initial moment of perfusion. There is evidence that the preferential binding of DMBA metabolites to matrix DNA is due to the increased accessibility of the DNA to the metabolites rather than to its proximity to the nuclear membrane. This accessibility, in its turn, is due to the peculiarities of the supranucleosomal structure of transcriptionally active DNP fragments associated with the nuclear matrix.
已证明与核基质紧密相关的DNA优先结合7,12-二甲基苯并(a)蒽（DMBA）代谢物。在灌注大鼠肝脏的实验中发现，DNP所有DNA组分中加合物形成的动力学曲线都有最大值，在灌注开始时观察到DNA组分中代谢物结合速率的最大差异。有证据表明，DMBA代谢物与基质DNA的优先结合是由于DNA对代谢物的可及性增加，而不是由于其与核膜的接近程度。反过来，这种可及性是由于与核基质相关的转录活性DNP片段的超核小体结构的特殊性。
