Ward L C, Buttery P J, Boorman K N
Int J Biochem. 1984;16(10):1077-81. doi: 10.1016/0020-711x(84)90091-0.
Protein synthesis was measured in perfused rat skeletal muscle using [14C]lysine in the presence of increasing amounts of lysine in the perfusate. The results obtained clearly indicated that both the extracellular and intracellular pools of amino acids act as direct precursors for protein synthesis and analysis of the data, according to O'Hara et al. (J. molec. cell. Cardiol. 13, 925-940, 1981) further indicated that extracellular sources of lysine supplied 64 and 81% of the lysine incorporated in the absence and presence of insulin, respectively. The rate of protein synthesis were assessed to be 0.22 mumol lysine incorporated g-1 muscle per 3 hr of perfusion increasing to 1.0 mumol g-1 3 hr-1 in the presence of insulin.
在灌注液中赖氨酸含量不断增加的情况下，使用[¹⁴C]赖氨酸对灌注的大鼠骨骼肌中的蛋白质合成进行了测量。根据奥哈拉等人（《分子与细胞心脏病学杂志》13卷，925 - 940页，1981年）的研究，所得结果清楚地表明，细胞外和细胞内的氨基酸池均作为蛋白质合成的直接前体，对数据的分析进一步表明，在不存在胰岛素和存在胰岛素的情况下，细胞外赖氨酸来源分别提供了掺入的赖氨酸的64%和81%。在胰岛素存在的情况下，蛋白质合成速率经评估为每3小时灌注每克肌肉掺入0.22微摩尔赖氨酸，增加至1.0微摩尔·克⁻¹·3小时⁻¹。
