Kreutzmann H L
Folia Haematol Int Mag Klin Morphol Blutforsch. 1976;103(5):686-700.
It was found that the granulocytes for Anguilla anguilla mainly form in the kidney. The stem cell of granulocytes is the large hemocytoblast. Sequences of development were established for the heterophil, cosinophil and basophil granulocytes. Myeloblasts, promyelocytes, metamyelocytes, mature red-shaped and segmented forms were described as stages of development of the heterophil granulocytes. In the case of the eosinophil and basophil granulocytes the myeloblastic and myelocytic stages could be demonstrated as well as the mature granulocyte. By the aid of special granular staining, phase contrast observations, supravital and cytochemical investigations the granulocytes could be described. The cytochemical proof for the granulocytes delivered a distribution pattern of the lipids, carbohydrates, RNA (RNS) and enzymes: unspecific esterase, alkaline and acid phosphatases, oxydase and peroxydase. By means of heart puncture a larger loss of blood was caused and the hematopoiesis stimulated. The following blood letting gave indications of how rapidly the granulopoiesis develops and how much time maturing takes. After a larger loss of blood the new-growth of the granulocytes will be completed nine days later.
研究发现，欧洲鳗鲡的粒细胞主要在肾脏形成。粒细胞的干细胞是大造血母细胞。确立了嗜异性粒细胞、嗜酸性粒细胞和嗜碱性粒细胞的发育序列。原粒细胞、早幼粒细胞、中幼粒细胞、成熟红细胞形和分叶形被描述为嗜异性粒细胞的发育阶段。对于嗜酸性粒细胞和嗜碱性粒细胞，可显示原粒细胞和中幼粒细胞阶段以及成熟粒细胞。借助特殊的颗粒染色、相差观察、超活染色和细胞化学研究，可对粒细胞进行描述。粒细胞的细胞化学证据给出了脂质、碳水化合物、RNA（核糖核酸）和酶的分布模式：非特异性酯酶、碱性和酸性磷酸酶、氧化酶和过氧化物酶。通过心脏穿刺造成较大的失血，并刺激造血。随后的放血显示了粒细胞生成发展的速度以及成熟所需的时间。失血较多后，粒细胞的新生将在九天后完成。
