Kreutzmann H L
Folia Haematol Int Mag Klin Morphol Blutforsch. 1976;103(2):226-35.
By means of heart tapping we produced a larger loss of blood in the European eel, Anguilla anguilla. This resulted in enriching the peripheric blood with erythroblasts and proerythrocytes. By panoptic staining erythroblasts, proerythrocytes and mature erythrocytes could be prepared and described. By the aid of supravital staining it was possible to detect mitochondria and Substantia granulofilamentosa in all stages of development. Phase contrast observations proved that the erythrocytes of Anguilla anguilla do not possess any locomotion ability. Cytochemical investigations showed that the erythrocytes weakly or very weakly react to acid phosphatases, lipids and ribonucleic acids and positively to unspecific esterases. The artificially produced loss of blood and the following continuous extraction of blood gave indications of how rapidly the erythrocytes form and how much time their maturing takes. 48 hours after tapping erythroblasts appeared in large amounts. The number of proerythrocytes increased 24 hours later. The transformation of proerythrocytes into mature erythrocytes does not take more than 48 hours. The loss of erythrocytes caused by heart tapping is already compensated six days later by new growth. During this investigation division processes in erythrocytes were observed. These are endomitotic or amitotic division or mitotic ones which are very rarely observed.
通过心脏穿刺放血的方法，我们在欧洲鳗鲡（Anguilla anguilla）身上造成了大量失血。这使得外周血中富含成红细胞和早幼红细胞。通过全景染色，可以制备并描述成红细胞、早幼红细胞和成熟红细胞。借助超活染色，能够在各个发育阶段检测到线粒体和颗粒丝状物质。相差显微镜观察证明，欧洲鳗鲡的红细胞不具备任何运动能力。细胞化学研究表明，红细胞对酸性磷酸酶、脂质和核糖核酸的反应微弱或非常微弱，而对非特异性酯酶呈阳性反应。人为造成的失血以及随后持续的采血显示了红细胞形成的速度有多快以及它们成熟需要多长时间。穿刺放血48小时后，大量成红细胞出现。24小时后，早幼红细胞数量增加。早幼红细胞向成熟红细胞的转化不超过48小时。心脏穿刺造成的红细胞损失在六天后已被新生成的红细胞所补偿。在这项研究中观察到了红细胞的分裂过程。这些是核内有丝分裂或无丝分裂，或者是非常罕见的有丝分裂。
