Kimber I, Bakács T, Roberts K, Moore M
J Clin Lab Immunol. 1984 Oct;15(2):77-84.
Supernatants derived from MLA-144, a gibbon T cell line that constitutively releases interleukin-2 (IL-2) and which lack detectable interferon (IFN) had the capacity to enhance the natural killer (NK) cells function of human peripheral blood lymphocytes. Percoll fractionation revealed that like IFN-inducible cytotoxic cells lymphocytes responding to MLA-144 supernatants cofractionate with native NK cells. Exposure to MLA-144 conditioned medium also potentiated the cytotoxic capacity of extravascular effector cells which are either unresponsive or weakly responsive to interferon-alpha (IFN-alpha). Moreover lymphocyte (K cell)-mediated antibody-dependent cellular cytotoxicity (ADCC) which is unresponsive to IFN-alpha was in most cases subject to a modest potentiation following treatment with MLA-144 supernatants. These data confirm previous reports that IL-2 can regulate peripheral blood NK cell function and also demonstrate that this lymphokine can also influence those natural cytotoxic mechanisms which are unresponsive to IFN-alpha.
从长臂猿T细胞系MLA - 144获得的上清液，该细胞系组成性释放白细胞介素 - 2（IL - 2）且检测不到干扰素（IFN），具有增强人外周血淋巴细胞自然杀伤（NK）细胞功能的能力。Percoll分级分离显示，与对IFN诱导的细胞毒性细胞一样，对MLA - 144上清液有反应的淋巴细胞与天然NK细胞共分级分离。暴露于MLA - 144条件培养基也增强了血管外效应细胞的细胞毒性能力，这些细胞对α - 干扰素（IFN - α）无反应或反应较弱。此外，对IFN - α无反应的淋巴细胞（K细胞）介导的抗体依赖性细胞毒性（ADCC）在大多数情况下，在用MLA - 144上清液处理后会有适度增强。这些数据证实了先前的报道，即IL - 2可以调节外周血NK细胞功能，并且还表明这种淋巴因子也可以影响那些对IFN - α无反应的天然细胞毒性机制。
