Woessner S, Lafuente R, Florensa L, Sans-Sabrafen J
Acta Haematol. 1984;72(5):303-8. doi: 10.1159/000206406.
We have studied the amount and intracellular distribution of erythroblastic alkaline and acid phosphatase, nonspecific esterase, and N-acetyl-beta-glucosaminidase in 50 patients with acquired dyserythropoiesis. 19 morphologically normal bone marrow smears served as controls. Alkaline phosphatase was found in all controls (mean 9.4% of erythroblasts). The percentage of positive erythroblasts in pathological conditions varied greatly from absence to over 70%. Acid phosphatase and N-acetyl-beta-glucosaminidase were positive in pernicious anaemia and in acute erythremic myelosis. Nonspecific esterase was only detected in a case of erythremic myelosis. This case also showed a faint metachromasia with the dye azure A.
我们研究了50例获得性红细胞生成异常患者中红系碱性磷酸酶、酸性磷酸酶、非特异性酯酶和N-乙酰-β-葡萄糖胺酶的含量及细胞内分布。19份形态学正常的骨髓涂片作为对照。在所有对照中均发现碱性磷酸酶(平均占幼红细胞的9.4%)。在病理情况下,阳性幼红细胞的百分比差异很大,从无到超过70%。酸性磷酸酶和N-乙酰-β-葡萄糖胺酶在恶性贫血和急性红白血病中呈阳性。非特异性酯酶仅在1例红白血病中检测到。该病例用天青A染料染色时也显示出微弱的异染性。