Korkeala H, Mäki-Petäys O
Acta Vet Scand. 1984;25(3):352-64. doi: 10.1186/BF03547250.
Various tissues and body fluids of pigs given chloramphenicol intramuscularly at a dose level of 20 mg/kg 1.5, 2.5 and 18 h before slaughter were examined for drug residues with different agar diffusion methods. Zones of inhibition were observed in bile, kidney, muscle, serum and urine samples 1.5 h after drug administration. After 19 h, residues were found only in the urine. The treatment of the bile, kidney, serum and urine samples with β-glucuronidase lowered the detection threshold of the agar diffusion methods for chloramphenicol. In addition, β-glucuronidase can be used for the identification of chloramphenicol residues. Chloramphenicol yielded the greatest zones of inhibition in kidney medulla and especially in urine with and without β-glucuronidase. 18 h after drug administration residues were found with β-glucuronidase treatment only in these samples. Urine and kidney medulla proved the best samples in the residue analysis of chloramphenicol at meat inspection.
在屠宰前1.5、2.5和18小时，给猪肌肉注射剂量为20mg/kg的氯霉素，然后用不同的琼脂扩散法检测猪的各种组织和体液中的药物残留。给药1.5小时后，在胆汁、肾脏、肌肉、血清和尿液样本中观察到抑菌圈。19小时后，仅在尿液中发现残留。用β-葡萄糖醛酸酶处理胆汁、肾脏、血清和尿液样本，降低了琼脂扩散法检测氯霉素的阈值。此外，β-葡萄糖醛酸酶可用于鉴定氯霉素残留。无论有无β-葡萄糖醛酸酶，氯霉素在肾髓质尤其是尿液中产生的抑菌圈最大。给药18小时后，仅在经β-葡萄糖醛酸酶处理的这些样本中发现残留。在肉类检验中，尿液和肾髓质被证明是氯霉素残留分析的最佳样本。
