Pouletty P, Pinon J M, Garcia-Gonzalez M, Desmonts G, Thulliez P, Thoannes H, Kadouche J
Eur J Clin Microbiol. 1984 Dec;3(6):510-5. doi: 10.1007/BF02013609.
An anti-human mu-chain monoclonal antibody, Tibi 82, was produced and tested for specificity by radioimmunoassay. Its reliability in detecting IgM antibodies to Toxoplasma gondii was tested by two reverse immunosorbent methods (IgM-ISAGA and IgM-SPIHA) and the IgM fluorescent antibody test (IgM-IFA) on 400 sera. Whereas the results obtained with Tibi 82 and with two polyclonal reagents were highly correlated, the third commercial polyclonal reagent provided many false negative results. By standardizing IgM binding, Tibi 82 allowed the comparison of IgM-ISAGA with IgM-SPIHA on 100 sera: 17% of the sera tested showed discrepancies due to the different toxoplasma antigens used. Although Tibi 82 facilitated the reading of results and enhanced sensitivity and specificity of the double-sandwich IgM-IFA method, the latter was still less sensitive than IgM-ISAGA with Tibi 82. Tests with the monoclonal antibody were consistently superior to tests with polyclonal antibodies.
制备了一种抗人μ链单克隆抗体Tibi 82，并通过放射免疫分析检测其特异性。采用两种反向免疫吸附法（IgM-ISAGA和IgM-SPIHA）以及IgM荧光抗体试验（IgM-IFA）对400份血清检测Tibi 82检测弓形虫IgM抗体的可靠性。尽管Tibi 82与两种多克隆试剂得到的结果高度相关，但第三种商用多克隆试剂却产生了许多假阴性结果。通过标准化IgM结合，Tibi 82可对100份血清进行IgM-ISAGA和IgM-SPIHA的比较：由于使用的弓形虫抗原不同，17%的检测血清显示出差异。尽管Tibi 82便于结果判读，并提高了双夹心IgM-IFA方法的敏感性和特异性，但后者仍不如使用Tibi 82的IgM-ISAGA敏感。单克隆抗体检测始终优于多克隆抗体检测。
