Aebersold R, Herbst H, Chang J Y, Braun D G
Hoppe Seylers Z Physiol Chem. 1984 Dec;365(12):1385-91. doi: 10.1515/bchm2.1984.365.2.1385.
Antibody 50S10.1 is a hybridoma-derived gamma 3 kappa antibody of BAB-14 mouse strain origin, with specificity for N-acetylglucosamine beta 1----3 linked to L-rhamnose, the immunodeterminant of the streptococcal Group A polysaccharide. The VL50S10.1 amino acid sequence is the fourth complete one reported with this specificity and the first fully determined V kappa 21A structure. Furthermore it is the first V kappa 21A isotype sequence derived from an antibody with known antigen specificity. The V kappa region of this and the previously described monoclonal anti-streptococcal Group A polysaccharide antibodies 7S34.1, 2S1.3 and 17S29.1 are compared, showing that in monoclonal antibody 50S10.1 a V kappa germline gene is expressed which is unrelated to those previously shown to be expressed in antibodies of this specificity. V kappa 50S10.1 increases the variability of known murine V kappa regions and confirms stretches of V kappa 21A sequences previously established.
抗体50S10.1是一种源自杂交瘤的γ3κ抗体，来源于BAB - 14小鼠品系，对与L - 鼠李糖相连的N - 乙酰葡糖胺β1----3具有特异性，这是A群链球菌多糖的免疫决定簇。VL50S10.1氨基酸序列是报道的具有这种特异性的第四个完整序列，也是第一个完全确定的Vκ21A结构。此外，它是第一个源自具有已知抗原特异性抗体的Vκ21A同种型序列。将此抗体与先前描述的单克隆抗A群链球菌多糖抗体7S34.1、2S1.3和17S29.1的Vκ区域进行比较，结果表明，在单克隆抗体50S10.1中表达的Vκ种系基因与先前在具有这种特异性的抗体中显示表达的基因无关。Vκ50S10.1增加了已知小鼠Vκ区域的变异性，并证实了先前确定的Vκ21A序列片段。
