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一种来自小鼠杂交瘤衍生的抗（A 群链球菌多糖）抗体的κ轻链可变区的新同种型序列（Vκ27），该抗体含有一个额外的半胱氨酸残基。应用二甲基氨基偶氮苯异硫氰酸酯技术分离肽段。

A new isotype sequence (V kappa 27) of the variable region of kappa-light chains from a mouse hybridoma-derived anti-(streptococcal group A polysaccharide) antibody containing an additional cysteine residue. Application of the dimethylaminoazobenzene isothiocyanate technique for the isolation of peptides.

作者信息

Chang J Y, Herbst H, Aebersold R, Braun D G

出版信息

Biochem J. 1983 Apr 1;211(1):173-80. doi: 10.1042/bj2110173.

DOI:10.1042/bj2110173
PMID:6409088
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1154341/
Abstract

The first complete sequence of the variable region of a kappa-light chain (V kappa) from a mouse anti-(streptococcal group A polysaccharide) antibody (immunoglobulin 7S34.1) is reported. Immunoglobulin 7S34.1 was isolated from the ascitic fluid of hybridoma 7S34.1 previously cloned in vitro. A newly developed technique for the isolation of peptides by using pre-column formation of peptide derivatives with dimethylaminoazobenzene isothiocyanate also served to complete the sequence. The sequence of the variable region of the kappa-light chain of immunoglobulin 7S34.1 defines a new mouse V kappa isotype (V kappa 27) and is the first mouse immunoglobulin light-chain variable region to be shown to have an extra cysteine residue at position 48.

摘要

报道了来自小鼠抗A组链球菌多糖抗体（免疫球蛋白7S34.1）的κ轻链可变区（Vκ）的首个完整序列。免疫球蛋白7S34.1是从先前体外克隆的杂交瘤7S34.1的腹水中分离出来的。一种通过使用二甲基氨基偶氮苯异硫氰酸酯进行柱前肽衍生物形成来分离肽的新开发技术也有助于完成该序列。免疫球蛋白7S34.1的κ轻链可变区序列定义了一种新的小鼠Vκ同种型（Vκ27），并且是首个被证明在第48位具有额外半胱氨酸残基的小鼠免疫球蛋白轻链可变区。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8b/1154341/fb20c2c5a0a5/biochemj00354-0180-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8b/1154341/fb20c2c5a0a5/biochemj00354-0180-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8a8b/1154341/fb20c2c5a0a5/biochemj00354-0180-a.jpg

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本文引用的文献

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Hoppe Seylers Z Physiol Chem. 1982 Sep;363(9):1069-76. doi: 10.1515/bchm2.1982.363.2.1069.
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J Virol. 1990 Mar;64(3):1171-81. doi: 10.1128/JVI.64.3.1171-1181.1990.
抗A组链球菌抗体：小鼠脾细胞体外激活与杂交后的产生
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