de Almeida J O
Mem Inst Oswaldo Cruz. 1984 Jul-Sep;79(3):363-7. doi: 10.1590/s0074-02761984000300010.
The aqueous extract of Trypanosoma cruzi, previously treated by benzene, was filtered through a column of Sephadex G-200. The eluted fractions were tested by complement-fixation with the chagasic reference serum. It was found that the CF antigens were eluted in the first fractions and the contaminants in the last ones. The aqueous antigen, when dried and extracted with methanol, showed a very high titer in the complement-fixation quantitative test. This antigen is free of contaminants and can be recommended for use in the serological test for the diagnosis of Chagas' disease.
经苯处理过的克氏锥虫水提取物通过Sephadex G - 200柱进行过滤。用恰加斯病参考血清通过补体结合试验对洗脱的组分进行检测。结果发现，补体结合抗原在最初的组分中被洗脱出来，而污染物在最后的组分中。干燥后的水性抗原用甲醇提取后，在补体结合定量试验中显示出非常高的滴度。这种抗原不含污染物，可推荐用于恰加斯病诊断的血清学检测。
