Matos A, Ramírez L E
Disciplina de Parasitologia, Faculdade de Medicina do Triângulo Mineiro, Uberaba, MG.
Rev Soc Bras Med Trop. 1997 Nov-Dec;30(6):493-9. doi: 10.1590/s0037-86821997000600009.
From this present data it has been evaluated a new complement fixation test, comparatively to indirect immunofluorescence to diagnose chronic Chagas' disease, utilizing one watery extract of epimastigotes of Trypanosoma cruzi and three other ethanolic extracts: one from epimastigotes, one from tripomastigotes and a third one of amastigotes obtained from cultures. Utilizing 236 serum samples indirect immunofluorescence test was performed: 109 positives (20 of them with positive parasitologic diagnostic) and 127 negatives (96 of healthy blood donors and 31 with other diseases). The results have showed that is possible a positive reaction in diluted samples up to 1:16. The best limits of reactivity found were the dilutions 1:4 for the ethanolic extract of amastigotes and 1:2 for the others antigens. The correlation index among the new complement fixation test and indirect immunofluorescence test showed that the ethanolic extract from epimastigotes was the best antigen to be utilized to diagnosis purposes. Its co-positivity index with indirect immunofluorescence was 0.92207 and the co-negative index was 0.90000. Concluding, the new complement fixation test showed itself as a fast, sensible, easily applicable semiquantitative microtechnique to the diagnosis of chronic Chagas' disease.
根据目前的数据，我们评估了一种新的补体结合试验，与间接免疫荧光法相比，用于诊断慢性恰加斯病，使用了克氏锥虫无鞭毛体的水性提取物和其他三种乙醇提取物：一种来自无鞭毛体，一种来自上鞭毛体，第三种来自培养获得的无鞭毛体。对236份血清样本进行了间接免疫荧光试验：109份阳性（其中20份寄生虫学诊断阳性）和127份阴性（96份为健康献血者，31份患有其他疾病）。结果表明，稀释至1:16的样本仍可能出现阳性反应。发现的最佳反应限度是无鞭毛体乙醇提取物的稀释度为1:4，其他抗原的稀释度为1:2。新的补体结合试验与间接免疫荧光试验之间的相关指数表明，上鞭毛体乙醇提取物是用于诊断目的的最佳抗原。其与间接免疫荧光的共同阳性指数为0.92207，共同阴性指数为0.90000。结论是，新的补体结合试验是一种快速、灵敏、易于应用的半定量微技术，可用于慢性恰加斯病的诊断。
