作为示踪剂的氟化分子：通过电子能量损失谱对人血小板中的二氟血清素进行映射分析。

Fluorinated molecule as a tracer: difluoroserotonin in human platelets mapped by electron energy-loss spectroscopy.

作者信息

Costa J L, Joy D C, Maher D M, Kirk K L, Hui S W

出版信息

Science. 1978 May 5;200(4341):537-9. doi: 10.1126/science.644312.

Abstract

The intracellular distribution of fluorine has been delineated in human platelets incubated with 4,6-difluoroserotonin, utilizing a scanning-transmission electron microscope equipped with an energy-loss spectrometer. Discrete intracellular structures corresponding in location to dense bodies contained high concentrations of fluorine. Electron energy-loss spectroscopy, which apparently can detect less than 10(-20) gram of fluorine in an area of 10 square nonometers, can thus localize fluorinated tracer molecules with biological activity.

摘要

利用配备能量损失谱仪的扫描透射电子显微镜，已描绘出在与4,6 - 二氟血清素一起孵育的人血小板中氟的细胞内分布情况。在位置上与致密体相对应的离散细胞内结构含有高浓度的氟。电子能量损失谱显然能够在10平方纳米的区域内检测到少于10^(-20)克的氟，因此可以定位具有生物活性的氟化示踪分子。