Weimer S L, Campeau J D, Marrs R P, Dizerega G S
J In Vitro Fert Embryo Transf. 1984 Dec;1(4):263-6. doi: 10.1007/BF01131626.
Recently, it has been shown that granulosa-cell secretion of plasminogen activator (PA) is responsive to luteinizing hormone (LH)/human chorionic gonadotropin (hCG) as well as follicle stimulating hormone (FSH) in the rat and the pig. Accordingly, we asked whether PA activity in follicular fluid from exogenously stimulated human follicles was different from that of normal cycles and whether or not these activities correlated with follicular maturation as determined by follicular fluid steroid concentration. Follicular aspirates were obtained from women who were participating in an in vitro fertilization protocol. Follicular fluid concentrations of estradiol and progesterone were determined by established radioimmunoassay. PA activity, determined using a modified indirect solid-phase radioassay, was significantly less in follicles from patients treated with human menopausal gonadotropin (hMG) plus clomiphene (P less than 0.05) compared to untreated patients or those receiving hMG or clomiphene alone. Correlations of PA activity and follicular fluid steroid concentrations demonstrated no significant correlation in samples from treated patients. In contrast, untreated spontaneously cycling patients had a significant (r = 0.89, P less than 0.05), positive correlation between follicular fluid estradiol levels. There was no correlation between PA activity and follicular fluid progesterone levels in any of the groups. These results suggest that a subtle balance in granulosa-cell secretion of PA and steroid exists, which appears to be disrupted by follicular hyperstimulation during treatment of patients participating in in vitro fertilization protocols.
最近研究表明，在大鼠和猪体内，颗粒细胞分泌的纤溶酶原激活物（PA）对促黄体生成素（LH）/人绒毛膜促性腺激素（hCG）以及促卵泡激素（FSH）有反应。因此，我们探讨了外源性刺激的人卵泡液中的PA活性是否与正常周期不同，以及这些活性是否与通过卵泡液类固醇浓度测定的卵泡成熟相关。卵泡抽吸物取自参与体外受精方案的女性。通过既定的放射免疫测定法测定卵泡液中雌二醇和孕酮的浓度。使用改良的间接固相放射测定法测定PA活性，结果显示，与未治疗患者或仅接受人绝经期促性腺激素（hMG）或克罗米芬治疗的患者相比，接受hMG加克罗米芬治疗的患者卵泡中的PA活性显著降低（P<0.05）。PA活性与卵泡液类固醇浓度的相关性表明，治疗患者的样本中无显著相关性。相比之下，未经治疗的自然周期患者卵泡液雌二醇水平之间存在显著的正相关（r = 0.89，P<0.05）。在任何组中，PA活性与卵泡液孕酮水平之间均无相关性。这些结果表明，颗粒细胞分泌PA和类固醇之间存在微妙的平衡，在参与体外受精方案的患者治疗期间，卵泡过度刺激似乎会破坏这种平衡。
