用福尔马林对哈布蛇（竹叶青蛇）毒液的出血毒素进行解毒过程中pH值和赖氨酸对类毒素免疫原性的影响。

Effects of pH and lysine during detoxification of a hemorrhagic principle of Habu snake (Trimeresurus flavoviridis) venom with formalin on the immunogenicity of the toxoid.

作者信息

Sadahiro S, Omori-Satoh T, Kondo S, Matuhasi T

出版信息

Jpn J Med Sci Biol. 1984 Oct-Dec;37(5-6):225-31. doi: 10.7883/yoken1952.37.225.

DOI:10.7883/yoken1952.37.225

Abstract

A hemorrhagic principle (HR1B) isolated from the venom of Habu snake (Trimeresurus flavoviridis) was detoxified with formalin at pH 5, 7 and 9. Higher pH shortened the period needed for detoxification and lowered the final formalin concentration required for complete detoxification. The extent of polymerization and the immunogenicity of the toxoid formed were dependent upon pH during detoxification; the lower the pH, the higher the extent of polymerization and the immunogenicity of the toxoid. The higher immunogenicity possessed by the toxoid with higher extent of polymerization was confirmed by fractionation of the toxoid prepared at pH 7 on Sephadex G-200 and by comparing the immunogenicity of the toxoid fractions. The presence of lysine during detoxification brought about a partially polymerized toxoid having new immunogenic characteristics and a higher immunogenicity.

摘要

从蝮蛇（竹叶青）毒液中分离出的一种出血毒素（HR1B），在pH值为5、7和9的条件下用福尔马林进行解毒处理。较高的pH值缩短了解毒所需的时间，并降低了完全解毒所需的最终福尔马林浓度。解毒过程中形成的类毒素的聚合程度和免疫原性取决于pH值；pH值越低，类毒素的聚合程度和免疫原性越高。通过在Sephadex G - 200上对pH值为7时制备的类毒素进行分级分离，并比较类毒素各组分的免疫原性，证实了聚合程度较高的类毒素具有更高的免疫原性。解毒过程中赖氨酸的存在导致形成了具有新免疫原特性和更高免疫原性的部分聚合类毒素。