Emery D, McCullagh P
Transplantation. 1980;29(1):17-22. doi: 10.1097/00007890-198001000-00004.
Leukocytes from chimeric calves invariably failed to mount a proliferative response in excess of control responses when cultured with cotwin cells as stimulators. The levels of cellular proliferation in control cultures of leukocytes from chimeric donors were so low as to exclude the occurrence of any auto-stimulation, and it was clear that the absence of excess proliferation in mixed cultures could not be attributed to elevated background levels. Attempts to restore reactivity in mixed leukocyte culture by removal of cell subpopulations from chimeric calf leukocytes and by modification of cell surfaces were unsuccessful. It was inferred that the unreactivity between chimeric cotwins manifest in the normal lymphocyte transfer and mixed leukocyte reactions was unlikely to be produced by humoral or cellular blocking mechanisms.
当用同卵双生小牛的细胞作为刺激物进行培养时，嵌合小牛的白细胞总是无法产生超过对照反应的增殖反应。来自嵌合供体的白细胞对照培养物中的细胞增殖水平非常低，以至于排除了任何自身刺激的发生，并且很明显混合培养物中缺乏过度增殖不能归因于背景水平的升高。通过从嵌合小牛白细胞中去除细胞亚群以及修饰细胞表面来恢复混合白细胞培养反应性的尝试均未成功。据推断，正常淋巴细胞转移和混合白细胞反应中嵌合同卵双生小牛之间的无反应性不太可能是由体液或细胞阻断机制引起的。
