Lombart C, Sturgess J, Schachter H
Biochim Biophys Acta. 1980 Apr 17;629(1):1-12. doi: 10.1016/0304-4165(80)90259-7.
Turpentine-induced inflammation in the rat caused a 1.6--2.3-fold increase in liver homogenate sialyl-, galactosyl- and N-acetylglucosaminyltransferase total and specific enzyme activities. Peak transferase activities were achieved at about 40 h after turpentine injection; the rise and fall of these activities corresponded to a similar rise and fall in serum haptoglobin levels. Sialyl- and N-acetylglucosaminyltransferase activities were measured in both liver homogenates and Golgi-enriched membranes at 24 h after turpentine injection; both total and specific enzyme activities doubled in the homogenates following turpentine treatment but in the Golgi-enriched membranes only the total enzyme activities doubled while the specific enzyme activities increased only by about 20%. These findings suggest that turpentine injection results in an increase of Golgi complex protein relative to total cellular protein. This conclusion was supported by electron microscopic studies of rat liver at various times after turpentine injection. The increased glycosylation potential of the liver and the proliferation of liver Golgi complex may play an important role in the turpentine-induced secretion of acute-phase glycoproteins.
松节油诱导的大鼠炎症使肝匀浆中唾液酸基转移酶、半乳糖基转移酶和N - 乙酰葡糖胺基转移酶的总酶活性和比酶活性增加了1.6至2.3倍。在注射松节油后约40小时达到转移酶活性峰值；这些活性的上升和下降与血清触珠蛋白水平的类似上升和下降相对应。在注射松节油后24小时，对肝匀浆和富含高尔基体的膜中的唾液酸基转移酶和N - 乙酰葡糖胺基转移酶活性进行了测定；松节油处理后，匀浆中的总酶活性和比酶活性均增加了一倍，但在富含高尔基体的膜中，只有总酶活性增加了一倍，而比酶活性仅增加了约20%。这些发现表明，注射松节油会导致高尔基体复合物蛋白相对于总细胞蛋白增加。这一结论得到了松节油注射后不同时间大鼠肝脏电子显微镜研究的支持。肝脏糖基化潜力的增加和肝脏高尔基体复合物的增殖可能在松节油诱导的急性期糖蛋白分泌中起重要作用。
