Guillou P J, Woodhouse L F, Davison A M, Giles G R
Biomedicine. 1980 Feb;32(1):11-7.
The enhanced in vitro proliferative response of peripheral mononuclear cells (PMNC) when pre-cultured for 24 hours prior to the addition of Concanavalin-A has been used as an indirect parameter of suppressor cell activity in healthy subjects and in patients undergoing chronic haemodialysis. The proliferative response of PMNC from haemodialysis patients is lower than that of control subjects when Con-A is added at the initiation of culture but approximates to that of normal subjects when the addition of Con-A was deferred for 24 hours. The Suppressor Indices of PMNC from haemodialysis patients were at least as great and sometimes greater than those of control subjects but the absolute T-cell counts were lower in haemodialysis patients than in controls. These results suggest that the relative energy of haemodialysis patients is partly attributable to T-cell depletion but this is accompanied by retention, and in some cases augmentation, of suppressor cell activity.
在添加刀豆蛋白-A之前将外周血单个核细胞（PMNC）预培养24小时后，其体外增殖反应增强，这已被用作健康受试者和接受慢性血液透析患者中抑制细胞活性的间接参数。当在培养开始时添加刀豆蛋白-A时，血液透析患者的PMNC增殖反应低于对照组，但当刀豆蛋白-A的添加推迟24小时时，其增殖反应接近正常受试者。血液透析患者的PMNC抑制指数至少与对照组一样大，有时甚至大于对照组，但血液透析患者的绝对T细胞计数低于对照组。这些结果表明，血液透析患者的相对能量部分归因于T细胞耗竭，但同时伴随着抑制细胞活性的保留，在某些情况下还会增强。
