ADP-核糖从烟酰胺腺嘌呤二核苷酸（NAD）转移至霍乱毒素：一个亚基充当催化剂和受体蛋白。
Transfer of ADP-ribose from NAD to choleragen: a subunit acts as catalyst and acceptor protein.
作者信息
Trepel J B, Chuang D M, Neff N H
出版信息
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5440-2. doi: 10.1073/pnas.74.12.5440.
Abstract
Choleragen selectively incorporates 3H from [3H]NAD labeled on the adenosine moiety and not 14C from [14C]NAD labeled on the nicotinamide moiety. This reaction does not require protein in addition to choleragen. Incorporation of isotope does not proceed at 4 degrees, requires dithiothreitol, is stable after extensive washing with cold trichloroacetic acid, and is decreased 80% by boiling in trichloroacetic acid. Studies with the A and B subunits of choleragen show that the A subunit catalyzes ADP-ribosylation and serves as an acceptor protein. The B subunit does not show catalytic or acceptor activity. We conclude that choleragen and its A subunit catalyze the hydrolysis of NAD and the enzymatic transfer of ADP-ribose to the A subunit.
摘要
霍乱毒素选择性地结合来自腺苷部分标记有[³H]NAD的³H，而不结合来自烟酰胺部分标记有[¹⁴C]NAD的¹⁴C。除霍乱毒素外，该反应不需要蛋白质。同位素的掺入在4℃下不进行，需要二硫苏糖醇，在用冷三氯乙酸大量洗涤后稳定，并且在三氯乙酸中煮沸会降低80%。对霍乱毒素A和B亚基的研究表明，A亚基催化ADP-核糖基化并作为受体蛋白。B亚基不显示催化或受体活性。我们得出结论，霍乱毒素及其A亚基催化NAD的水解以及ADP-核糖向A亚基的酶促转移。
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