Hurle J M, Icardo J M, Ojeda J L
J Embryol Exp Morphol. 1980 Apr;56:211-23.
The hearts of chick embryos of stages 9-13 were subjected to SEM, TEM and histochemical studies to ascertain possible regional differences in the structure and composition of the cardiac jelly. Two distinct regions, the cardiac jelly filling the space located between the myocardium and the endocardium (MECJ) and the cardiac jelly filling the dorsal mesocardium (EECJ), were distinguished by their structural and histochemical properties. MECJ is formed by amorphous and fibrillar material arranged between the endocardial and myocardial layer. The amount of its components increases when cetylpyridinium chloride is introduced into the fixative, and it appears intensely stained by ruthenium red and alcian blue at low concentrations of MgCl2. The amount and arrangement of its components increase during the beginning of the looping process of the heart tube. The EECJ is very rich in ruthenium-red-positive basal-lamina-like material and the addition of cetylpyridinium chloride to the fixative does not modify its appearance. It also appears poorly stained by alcian blue at low concentrations of MgCl2 and its arrangment undergoes modifications closely associated with the events of endocardial fusion. The possible significance of these results in the early morphogenesis of the heart is discussed.
对9至13期鸡胚心脏进行扫描电子显微镜（SEM）、透射电子显微镜（TEM）和组织化学研究，以确定心胶在结构和组成上可能存在的区域差异。通过结构和组织化学特性区分出两个不同区域，即填充心肌层和心内膜之间间隙的心胶（MECJ）以及填充背侧心内膜的心胶（EECJ）。MECJ由排列在内皮和心肌层之间的无定形和纤维状物质形成。当将十六烷基氯化吡啶引入固定剂时，其成分数量增加，并且在低浓度MgCl2下，它被钌红和阿尔辛蓝强烈染色。在心脏管开始形成袢的过程中，其成分的数量和排列增加。EECJ富含钌红阳性的基底膜样物质，向固定剂中添加十六烷基氯化吡啶不会改变其外观。在低浓度MgCl2下，它也被阿尔辛蓝染色较差，并且其排列与心内膜融合事件密切相关的变化。讨论了这些结果在心脏早期形态发生中的可能意义。
