Nakamura A, Manasek F J
J Embryol Exp Morphol. 1978 Feb;43:167-83.
The properties of the early chick embryonic heart cardiac jelly were studied. The cells of the heart were removed by sequential treatments with calcium magnesium-free medium; the same medium containing 5 mM EDTA; and aqueous 0.1% deoxycholate. The transparent, naked cardiac jelly retained the original shape and size of the untreated original heart when immersed in physiological ionic strength medium. Its size and shape responded to changes in the ionic strength of the surrounding media. Alcian blue, cetylpyridinium chloride and testicular hyaluronidase abolished the ability of the jelly to respond to ionic strength changes. Electron microscope examination of the negatively stained spread cardiac jelly revealed an extensive network of collagenous fibrils and fine filaments with some amorphous adhering material. Treatment with testicular hyaluronidase removed much of the amorphous material and improved the details of the filaments. These results suggest that glycosaminoglycans play an important part in the hydration of the cardiac jelly and that the stability of the cardiac jelly shape is mainly due to the filamentous network and their possible interactions with macromolecules of the cardiac jelly matrix. It is suggested that the factors that control the depositon of the connective tissue macromolecules and the assembly of the filamentous network are significant factors which influence the morphogenesis of the early embryonic heart.
对早期鸡胚心脏的心胶特性进行了研究。通过依次用无钙镁培养基、含5 mM乙二胺四乙酸（EDTA）的相同培养基以及0.1%脱氧胆酸钠水溶液处理，去除心脏细胞。当浸入生理离子强度培养基中时，透明、裸露的心胶保留了未处理原始心脏的原始形状和大小。其大小和形状会对周围培养基离子强度的变化做出反应。阿尔辛蓝、十六烷基氯化吡啶和睾丸透明质酸酶消除了心胶对离子强度变化做出反应的能力。对经负染展开的心胶进行电子显微镜检查，发现有广泛的胶原纤维和细丝网络，伴有一些无定形附着物质。用睾丸透明质酸酶处理可去除大部分无定形物质，并改善细丝的细节。这些结果表明，糖胺聚糖在心脏胶状物的水合作用中起重要作用，并且心脏胶状物形状的稳定性主要归因于丝状网络及其与心脏胶状物基质大分子的可能相互作用。有人提出，控制结缔组织大分子沉积和丝状网络组装的因素是影响早期胚胎心脏形态发生的重要因素。
