Ohtsuki K, Ishida N
J Antibiot (Tokyo). 1980 Jul;33(7):744-50. doi: 10.7164/antibiotics.33.744.
The biological effects of both nonprotein component (NPC) and PC (protein component) from NCS have been studied in vivo and in vitro. NPC was found to not only inhibit DNA synthesis in growing cells but also induce DNA degradation in vivo and in vitro. However, neither these two biological activities of PC were detected even at a 100-times higher concentration of NPC (0.2 micrograms/ml) which inhibited 50% DNA synthesis in growing cells. NPC-induced DNA degradation in vitro was stimulated by 2-mercaptoethanol as has been reported for NCS. These results show that the NPC removed from NCS is responsible for the biological activities such as the inhibition of DNA synthesis in growing cells and the induction of DNA degradation in vivo and in vitro.
已在体内和体外研究了来自NCS的非蛋白质成分（NPC）和PC（蛋白质成分）的生物学效应。发现NPC不仅抑制生长细胞中的DNA合成，还在体内和体外诱导DNA降解。然而，即使在抑制生长细胞中50%DNA合成的NPC浓度（0.2微克/毫升）高100倍的情况下，也未检测到PC的这两种生物学活性。如之前关于NCS的报道，2-巯基乙醇可刺激NPC在体外诱导的DNA降解。这些结果表明，从NCS中去除的NPC是导致诸如抑制生长细胞中的DNA合成以及在体内和体外诱导DNA降解等生物学活性的原因。
