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大肠杆菌中purF-lac融合及purF转录方向

purF-lac fusion and direction of purF transcription in Escherichia coli.

作者信息

Smith J M, Gots J S

出版信息

J Bacteriol. 1980 Sep;143(3):1156-64. doi: 10.1128/jb.143.3.1156-1164.1980.

DOI:10.1128/jb.143.3.1156-1164.1980
PMID:6447689
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC294467/
Abstract

The purF locus codes for the first enzyme, glutamine phosphoribosylpyrophosphate amidotransferase, of the purine biosynthetic pathway. A strain of Escherichia coli K-12 was isolated in which the lac structural genes were fused to the control region of the purF locus. This purF-lac fusion was shown to respond to purine-specific regulatory signals. A plaque-forming lambda transducing phage bearing this purF-lac fusion was isolated. This phage was used to genetically determine the direction of transcription for the pufF locus by two independent means. Results from both methods agreed that the direction of transcription of the purF locus was clockwise on the standard Escherichia coli K-12 genetic map.

摘要

purF基因座编码嘌呤生物合成途径中的第一种酶，即谷氨酰胺磷酸核糖焦磷酸酰胺转移酶。分离出了一株大肠杆菌K - 12菌株，其中乳糖结构基因与purF基因座的控制区域融合。已证明这种purF - lac融合对嘌呤特异性调节信号有反应。分离出了携带这种purF - lac融合的噬菌斑形成λ转导噬菌体。该噬菌体通过两种独立的方法用于从遗传学上确定purF基因座的转录方向。两种方法的结果均一致表明，在标准的大肠杆菌K - 12遗传图谱上，purF基因座的转录方向是顺时针的。

相似文献

1
purF-lac fusion and direction of purF transcription in Escherichia coli.大肠杆菌中purF-lac融合及purF转录方向
J Bacteriol. 1980 Sep;143(3):1156-64. doi: 10.1128/jb.143.3.1156-1164.1980.
2
Nucleotide sequence of Escherichia coli purF and deduced amino acid sequence of glutamine phosphoribosylpyrophosphate amidotransferase.大肠杆菌嘌呤F的核苷酸序列及谷氨酰胺磷酸核糖焦磷酸酰胺转移酶的推导氨基酸序列。
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Amino-terminal deletions define a glutamine amide transfer domain in glutamine phosphoribosylpyrophosphate amidotransferase and other PurF-type amidotransferases.氨基末端缺失确定了谷氨酰胺磷酸核糖焦磷酸酰胺转移酶及其他PurF型酰胺转移酶中的谷氨酰胺酰胺转移结构域。
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Regulation of expression of the dadA gene encoding D-amino acid dehydrogenase in Escherichia coli: analysis of dadA-lac fusions and direction of dadA transcription.大肠杆菌中编码D-氨基酸脱氢酶的dadA基因表达的调控：dadA-lac融合体分析及dadA转录方向
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引用本文的文献

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Role of hypoxanthine and guanine in regulation of Salmonella typhimurium pur gene expression.次黄嘌呤和鸟嘌呤在鼠伤寒沙门氏菌嘌呤基因表达调控中的作用。
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Isolation and characterization of regulatory mutations affecting the expression of the guaBA operon of Escherichia coli K-12.影响大肠杆菌K-12guaBA操纵子表达的调控突变的分离与鉴定。
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Linkage map of Escherichia coli K-12, edition 7.大肠杆菌K-12连锁图谱，第7版。
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J Bacteriol. 1990 Jun;172(6):3512-4. doi: 10.1128/jb.172.6.3512-3514.1990.

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