大肠杆菌中purF-lac融合及purF转录方向
purF-lac fusion and direction of purF transcription in Escherichia coli.
作者信息
Smith J M, Gots J S
出版信息
J Bacteriol. 1980 Sep;143(3):1156-64. doi: 10.1128/jb.143.3.1156-1164.1980.
Abstract
The purF locus codes for the first enzyme, glutamine phosphoribosylpyrophosphate amidotransferase, of the purine biosynthetic pathway. A strain of Escherichia coli K-12 was isolated in which the lac structural genes were fused to the control region of the purF locus. This purF-lac fusion was shown to respond to purine-specific regulatory signals. A plaque-forming lambda transducing phage bearing this purF-lac fusion was isolated. This phage was used to genetically determine the direction of transcription for the pufF locus by two independent means. Results from both methods agreed that the direction of transcription of the purF locus was clockwise on the standard Escherichia coli K-12 genetic map.
摘要
purF基因座编码嘌呤生物合成途径中的第一种酶，即谷氨酰胺磷酸核糖焦磷酸酰胺转移酶。分离出了一株大肠杆菌K - 12菌株，其中乳糖结构基因与purF基因座的控制区域融合。已证明这种purF - lac融合对嘌呤特异性调节信号有反应。分离出了携带这种purF - lac融合的噬菌斑形成λ转导噬菌体。该噬菌体通过两种独立的方法用于从遗传学上确定purF基因座的转录方向。两种方法的结果均一致表明，在标准的大肠杆菌K - 12遗传图谱上，purF基因座的转录方向是顺时针的。
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