Lee J H, Al-Zarban S, Wilcox G
J Bacteriol. 1981 Apr;146(1):298-304. doi: 10.1128/jb.146.1.298-304.1981.
Six L-arabinose transport-deficient mutants of Salmonella typhimurium LT2 were isolated on the basis of their inability to ferment low concentrations of L-arabinose. The mutations were localized between serA and lys on the S. typhimurium genetic map and assigned to the araE locus. An araE-lac fusion strain was constructed and used to determine that the direction of araE transcription was counterclockwise on the S. typhimurium genetic map. beta-Galactosidase activity was induced by L-arabinose in the araE-lac fusion strain, suggesting that araE expression is controlled at the level of transcription.
基于鼠伤寒沙门氏菌LT2无法发酵低浓度L-阿拉伯糖，分离出了六个L-阿拉伯糖转运缺陷型突变体。这些突变位于鼠伤寒沙门氏菌遗传图谱上的serA和lys之间，并被定位到araE基因座。构建了一个araE-lac融合菌株，并用于确定araE转录方向在鼠伤寒沙门氏菌遗传图谱上是逆时针方向。在araE-lac融合菌株中，L-阿拉伯糖可诱导β-半乳糖苷酶活性，这表明araE的表达在转录水平受到调控。
