Tsuji T, Araki K, Inoue J, Onoue K, Tsuchiya M, Shinohara T, Nagashima H, Nakashima A, Naito K, Arborgh B
Acta Med Okayama. 1980 Jun;34(3):209-16. doi: 10.18926/AMO/30553.
Immune complexes in liver specimens from 10 patients with chronic liver diseases [2 with chronic persistent hepatitis (CPH), 3 with chronic aggressive hepatitis (CAH) of moderate activity, 3 with CAH of severe activity, and 2 with liver cirrhosis] were examined by a technique of direct immunofluorescence using FITC-labelled human purified Clq (FITC-Clq). FITC-Clq bound to the nuclei of all cells in liver tissue. After DNase treatment, positive nuclei were absent, but positive staining with FITC-Clq remained in amorphous deposits and hepatic cell membranes in the areas of piecemeal necrosis of four CAH patients. Since FITC-Clq could not be demonstrated in the liver tissue of CPH and liver cirrhosis which contained no piecemeal necrosis, positive fluorescence in the liver of CAH patients was thought to indicate immune complexes bound to FITC-Clq. The fact that these positive substances, however, were few in number, may be the result of physiological mechanisms of immune clearance which rapidly eliminate immune complexes from the body.
采用异硫氰酸荧光素标记的人纯化C1q（FITC-C1q）直接免疫荧光技术，检测了10例慢性肝病患者肝组织中的免疫复合物[2例慢性持续性肝炎（CPH），3例中度活动的慢性侵袭性肝炎（CAH），3例重度活动的CAH，2例肝硬化]。FITC-C1q与肝组织中所有细胞的细胞核结合。经DNA酶处理后，阳性细胞核消失，但在4例CAH患者的碎片状坏死区域，FITC-C1q的阳性染色仍存在于无定形沉积物和肝细胞膜中。由于在无碎片状坏死的CPH和肝硬化肝组织中未检测到FITC-C1q，因此CAH患者肝脏中的阳性荧光被认为表明免疫复合物与FITC-C1q结合。然而，这些阳性物质数量很少，这可能是免疫清除的生理机制迅速从体内清除免疫复合物的结果。
