Nowoslawski A, Krawczyński K, Brzosko W J, Madaliński K
Am J Pathol. 1972 Jul;68(1):31-56.
In a significant percentage of examined cases of fulminant hepatitis, subacute hepatitis, chronic aggressive hepatitis, liver cirrhosis and chronic persistent hepatitis, Australia (hepatitis-associated) antigen (Au HAA) was identified in the liver and in extrahepatic locations. The several immunofluorescent patterns of Au HAA localization in hepatocytes strongly suggested various stages of Au HAA accumulation and release. Deposits of a mixture of immunoglobulins G and M and occasionally beta1C-globulin were found in the cytoplasm of Au HAA containing hepatocytes, on their plasma membranes, on or in the nuclei, in the cytoplasm of Kupffer cells and, rarely, in the sinusoids. The accompanying tissue changes were hepatocellular degeneration and necrosis. These intra- and extracellular complexes of Au HAA and immunoglobulins displayed strong affinity for guinea pig complement in the immunohistochemical complement fixation reaction. When tested by immunodiffusion in agar, IgG dissociated from these complexes by potassium thiocyanate (KSCN) treatment showed anti-Au HAA specificity. In fulminant hepatitis neither Au HAA nor immunoglobulins and complement were found in the liver. In chronic aggressive hepatitis and subacute hepatitis the amount of the Au HAA immune complexes identified in the liver was approximately inversely proportional to the extent and severity of the parenchymal lesions. In liver cirrhosis and chronic persistent hepatitis there was a positive correlation between the amount of the Au HAA immune complexes found in the liver and the degree of hepatocellular damage. The deposits of Au HAA, identified in extrahepatic locations including germinal centers of lymph nodes and spleen, kidney glomeruli and blood vessel walls, were as a rule accompanied by deposits of IgG, IgM, beta1C-globulin and fibrin. All these deposits showed strong affinity for guinea pig complement in the immunohistochemical reaction of complement fixation. Germinal center activation, chronic membraneous glomerulonephritis, panarteritis and simple arteriolar hyalinosis were found at sites of localization of these deposits.
在相当比例的暴发性肝炎、亚急性肝炎、慢性活动性肝炎、肝硬化和慢性持续性肝炎的检查病例中，在肝脏和肝外部位发现了澳大利亚（肝炎相关）抗原（Au HAA）。Au HAA在肝细胞中的几种免疫荧光模式强烈提示了Au HAA积累和释放的不同阶段。在含有Au HAA的肝细胞的细胞质、质膜上、核内或核上、库普弗细胞的细胞质中，偶尔也在肝血窦中，发现了免疫球蛋白G和M以及偶尔的β1C球蛋白的混合物沉积。伴随的组织变化是肝细胞变性和坏死。在免疫组织化学补体固定反应中，这些Au HAA和免疫球蛋白的细胞内和细胞外复合物对豚鼠补体显示出很强的亲和力。当通过琼脂免疫扩散进行检测时，经硫氰酸钾（KSCN）处理从这些复合物中解离的IgG显示出抗Au HAA特异性。在暴发性肝炎中，肝脏中未发现Au HAA、免疫球蛋白和补体。在慢性活动性肝炎和亚急性肝炎中，肝脏中鉴定出的Au HAA免疫复合物的量与实质病变的范围和严重程度大致成反比。在肝硬化和慢性持续性肝炎中，肝脏中发现的Au HAA免疫复合物的量与肝细胞损伤程度之间存在正相关。在包括淋巴结和脾脏的生发中心、肾小球和血管壁在内的肝外部位发现的Au HAA沉积物，通常伴有IgG、IgM、β1C球蛋白和纤维蛋白的沉积。在这些沉积物的定位部位发现了生发中心活化、慢性膜性肾小球肾炎、全动脉炎和单纯小动脉玻璃样变。
