van Heerde P, Feltkamp C A, Feltkamp-Vroom T M, Koudstaal J, van Unnik J A
Cancer. 1980 Nov 15;46(10):2210-20. doi: 10.1002/1097-0142(19801115)46:10<2210::aid-cncr2820461018>3.0.co;2-f.
From 74 patients with non-Hodgkin's lymphoma (NHL), fresh biopsy material from involved tissue was investigated histochemically and by light and electron microscopy. The results were compared with the light microscopical conclusions based on three currently utilized classifications, namely of Rappaport, Lukes, and Lennert. The separate groups of low-grade NHL appeared to consist of homogeneous cell populations, both in immunohistological as well as in enzymehistochemical and electron microscopical sense. On the contrary, high-grade NHL constituted a heterogeneous group in which supplementary (immunohistochemical, enzymehistochemical, and electron microscopical) investigation is very useful. All NHLs reacted with anti-human lymphocyte serum, including the so-called histiocytic NHLs according to the Rappaport classification, which proves their lymphocytic origin. Consequently the prefix "histiocytic" should be altered in a morphogenetically correct way. Without exception the B-cell NHLs were characterized by the presence of monoclonal immunoglobulin on the cell membrane and/or intracytoplasmically. In a part of the immunologically non-T-/non-B-/("null cell") NHLs, a B-cell origin was indicated by the presence of ATPase and/or 5' nucleotidase and a T-cell origin by the presence of dot-like acid phosphatase.
对74例非霍奇金淋巴瘤（NHL）患者受累组织的新鲜活检材料进行了组织化学、光镜和电镜检查。将结果与基于目前使用的三种分类法（即拉帕波特分类法、卢克斯分类法和伦纳特分类法）得出的光镜结论进行了比较。在免疫组织学、酶组织化学和电镜检查方面，低度NHL的各个亚组似乎都由同质的细胞群体组成。相反，高度NHL构成了一个异质性群体，补充（免疫组织化学、酶组织化学和电镜）检查非常有用。所有NHL均与抗人淋巴细胞血清发生反应，包括拉帕波特分类法中所谓的组织细胞性NHL，这证明了它们的淋巴细胞起源。因此，“组织细胞性”这一前缀应以形态发生学上正确的方式进行更改。无一例外，B细胞NHL的特征是细胞膜和/或细胞质内存在单克隆免疫球蛋白。在一部分免疫非T/非B/（“裸细胞”）NHL中，ATP酶和/或5'核苷酸酶的存在表明其起源于B细胞，点状酸性磷酸酶的存在表明其起源于T细胞。
