Kim H, Pangalis G A, Payne B C, Kadin M E, Rappaport H
Am J Pathol. 1982 Feb;106(2):204-23.
We applied a newly developed ultrastructural cytochemical technique utilizing 2-naphthyl thiol acetate (NTA) as a substrate in 3 cases of true histiocytic-monocytic malignancy and 3 controls. The enzyme NTA esterase was identified in both neoplastic and benign histiocytes-monocytes in the form of electron-dense material within the cytoplasm. The NTA esterase varied in size and shape and showed no clear relationship with cytoplasmic organelles. The distribution and pattern of staining were very similar to those of the light-microscopically demonstrated alpha-naphthyl acetate esterase reaction. The main advantages of this method are: 1) histiocytes-monocytes can be identified in the absence of lysosomes or phagocytosis; 2) unequivocal, simultaneous documentation of both histiocytic esterase and subcellular structure is possible; and 3) both blood and solid tissue specimens can be utilized. Our findings demonstrate the usefulness and applicability of ultrastructurally demonstrated NTA esterase in the study of histiocytic-monocytic malignancies.
我们应用一种新开发的超微结构细胞化学技术，以2-萘硫醇乙酸酯（NTA）为底物，对3例真性组织细胞-单核细胞恶性肿瘤患者和3例对照进行了研究。在肿瘤性和良性组织细胞-单核细胞中均鉴定出NTA酯酶，其表现为细胞质内电子致密物质的形式。NTA酯酶在大小和形状上各不相同，且与细胞质细胞器无明显关系。染色的分布和模式与光镜下显示的α-萘乙酸酯酶反应非常相似。该方法的主要优点是：1）在不存在溶酶体或吞噬作用的情况下可以识别组织细胞-单核细胞；2）可以明确、同时记录组织细胞酯酶和亚细胞结构；3）血液和实体组织标本均可使用。我们的研究结果证明了超微结构显示的NTA酯酶在组织细胞-单核细胞恶性肿瘤研究中的实用性和适用性。
