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苜蓿中华根瘤菌噬菌体16-3的杂合性:错配修复或基因转换的中等水平

Heterozygosis of phage 16-3 of Rhizobium meliloti: moderate level of mismatch repair or gene conversion.

作者信息

Orosz L, Páy A, Dallmann G

出版信息

Mol Gen Genet. 1980;179(1):163-7. doi: 10.1007/BF00268459.

DOI:10.1007/BF00268459
PMID:6450309
Abstract

Analysis of clear/turbid mottled (heterozygotic plaques) of Rhizobium meliloti temperate phage 16-3 indicates that the efficiency of repair at three sites (ti3, ti4, and ti5) in the C cistron is 2 to 20-fold less than that observed in E. coli phage lambda. In agreement with this conclusion, heterozygotic plaques were observed at similar frequency in crosses where point and small deletion mutants were combined, suggesting that in Rhizobium, DNA molecules with short single-stranded loops can escape from repair as efficiently as the simple mismatches.

摘要

苜蓿中华根瘤菌温和噬菌体16 - 3的清亮/浑浊斑驳(杂合噬菌斑)分析表明,C顺反子中三个位点(ti3、ti4和ti5)的修复效率比在大肠杆菌噬菌体λ中观察到的低2至20倍。与这一结论一致的是,在点突变体和小缺失突变体组合的杂交中,观察到杂合噬菌斑的频率相似,这表明在根瘤菌中,具有短单链环的DNA分子能够像简单错配一样有效地逃避修复。

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本文引用的文献

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Genetics. 1980 Feb;94(2):265-76. doi: 10.1093/genetics/94.2.265.
2
A comparison of two-point, three-point and deletion mapping in the C cistron of rhizobiophage 16-3, with an explanation for the recombination pattern.两种、三种和缺失作图法在根瘤噬菌 16-3 的 C 顺反子上的比较,以及对重组模式的解释。
Genetics. 1980 Feb;94(2):249-63. doi: 10.1093/genetics/94.2.249.
3
Localized Negative Interference in Bacteriophage.
DNA异源性对噬菌体λ重组的影响。
Genetics. 1988 Jan;118(1):13-9. doi: 10.1093/genetics/118.1.13.
4
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Genetics. 1980 Oct;96(2):321-9. doi: 10.1093/genetics/96.2.321.
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Possible correlation between transformability and deficiency in error-prone repair.可转化性与易错修复缺陷之间可能存在的相关性。
J Bacteriol. 1983 May;154(2):995-7. doi: 10.1128/jb.154.2.995-997.1983.
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Evidence for inclusion of regions of nonhomology in heteroduplex products of bacteriophage lambda recombination.噬菌体λ重组异源双链产物中包含非同源区域的证据。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7180-4. doi: 10.1073/pnas.81.22.7180.
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Effects of nonhomology on bacteriophage lambda recombination.非同源性对噬菌体λ重组的影响。
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噬菌体中的局部负干扰
Genetics. 1965 Mar;51(3):369-79. doi: 10.1093/genetics/51.3.369.
4
Genetic mapping of rhizobiophage 16-3.根瘤菌噬菌体16-3的遗传图谱
Acta Microbiol Acad Sci Hung. 1970;17(2):185-94.
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Genetic studies on rhizobiophage 16-3. I. Genes and functions on the chromosome.根瘤菌噬菌体16-3的遗传学研究。I. 染色体上的基因与功能
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