Helderman J H, Strom T B, Garovoy M R
J Clin Invest. 1981 Feb;67(2):509-13. doi: 10.1172/JCI110060.
Responses in the mixed lymphocyte culture (MLC) are traditionally evaluated by measurement of DNA synthesis or blast transformation. However, these events occur too late in the MLC to permit prospective matching for cadaveric renal transplantation. Presentation of allogeneic cells to the T lymphocyte within the MLC results in the emergence of an insulin receptor pharmacokinetically similar to that on other tissues such as fat, liver, and muscle. Intrafamilial MLC were studied by simultaneous assessment of DNA synthesis and insulin receptor binding. In 68 studies from seven families that provide examples of two haplotype identical matches, haplo-identical matches and total haplo mismatches, the presence of an insulin receptor correlated in every case with a positive MLC as estimated by [3H]thymidine incorporation. A quantitative relationship existed between the strength of the MLC and the amount of receptor binding. Based on analysis of cells from several families in which crossover events were known to have occurred, the appearance of an insulin receptor always corresponded with a mismatch at the portion of histocompatibility leukocyte antigen (HLA) chromosome bearing the D region. Finally, it was demonstrated in each of 30 cultures that insulin receptor emergence occurred significantly before detectable DNA synthesis, as early as 24 h after the initiation of the MLC, well within the time-constraint limitations for renal preservation. Appearance of the insulin receptor on activated lymphocytes may be a more rapid measure of mixed lymphocyte responses, and should permit prospective matching for cadaveric renal transplantation.
混合淋巴细胞培养（MLC）中的反应传统上是通过测量DNA合成或母细胞转化来评估的。然而，这些事件在MLC中发生得太晚，无法用于尸体肾移植的前瞻性配型。在MLC中，同种异体细胞呈递给T淋巴细胞会导致出现一种胰岛素受体，其药代动力学与脂肪、肝脏和肌肉等其他组织上的胰岛素受体相似。通过同时评估DNA合成和胰岛素受体结合来研究家族内MLC。在来自7个家族的68项研究中，这些研究提供了两个单倍型完全匹配、单倍型相同匹配和完全单倍型不匹配的例子，胰岛素受体的存在在每种情况下都与通过[3H]胸腺嘧啶核苷掺入估计的阳性MLC相关。MLC的强度与受体结合量之间存在定量关系。基于对几个已知发生了交叉事件的家族的细胞分析，胰岛素受体的出现总是与携带D区的组织相容性白细胞抗原（HLA）染色体部分的不匹配相对应。最后，在30个培养物中的每一个中都证明，胰岛素受体的出现明显早于可检测到的DNA合成，早在MLC开始后24小时，这完全在肾脏保存的时间限制范围内。活化淋巴细胞上胰岛素受体的出现可能是混合淋巴细胞反应更快的测量方法，并且应该允许进行尸体肾移植的前瞻性配型。
